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Journal of Virology, March 2003, p. 3301-3306, Vol. 77, No. 5
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.5.3301-3306.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Inhibition of Gammaherpesvirus Replication by RNA Interference

Qingmei Jia and Ren Sun*

Department of Molecular and Medical Pharmacology, AIDS Institute, Jonsson Comprehensive Cancer Center, Dental Research Institute, and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095

Received 29 August 2002/ Accepted 25 October 2002

RNA interference (RNAi) is a conserved mechanism in which double-stranded, small interfering RNAs (siRNAs) trigger a sequence-specific gene-silencing process. Here we describe the inhibition of murine herpesvirus 68 replication by siRNAs targeted to sequences encoding Rta, an immediate-early protein known as an initiator of the lytic viral gene expression program, and open reading frame 45 (ORF 45), a conserved viral protein. Our results suggest that RNAi can block gammaherpesvirus replication and ORF 45 is required for efficient viral production.

* Corresponding author. Mailing address: Department of Molecular and Medical Pharmacology, University of California at Los Angeles, Los Angeles, CA 90095. Phone: (310) 794-5557. Fax: (310) 794-5123. E-mail: rsun{at}mednet.ucla.edu.

Journal of Virology, March 2003, p. 3301-3306, Vol. 77, No. 5
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.5.3301-3306.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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