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Journal of Virology, March 2003, p. 2946-2955, Vol. 77, No. 5
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.5.2946-2955.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Exonuclease-Deficient Polymerase Mutant of Herpes Simplex Virus Type 1 Induces Altered Spectra of Mutations

Ying T. Hwang and Charles B. C. Hwang*

Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, New York 13210

Received 14 October 2002/ Accepted 27 November 2002

The effect of exonuclease activity of the herpes simplex virus DNA polymerase (Pol) on DNA replication fidelity was examined by using the supF mutagenesis assay. The recombinants with exonuclease-deficient Pol, containing an integrated supF gene in the thymidine kinase locus (tk), exhibited supF mutation frequencies ranging from 0.14 to 5.6%, consistent with the tk mutation frequencies reported previously (Y. T. Hwang, B.-Y. Liu, D. M. Coen, and C. B. C. Hwang, J. Virol. 71:7791-7798, 1997). The increased mutation frequencies were 10- to 500-fold higher than those observed for wild-type Pol recombinants. The increased mutation frequencies also were significantly higher than those of supF mutant replicated by exonuclease-deficient Pols in the plasmid-borne assay. Furthermore, characterization of supF mutants demonstrated that recombinants with a defective exonuclease induced types and distributions of supF mutations different from those induced by wild-type Pol recombinants. The types of supF mutations induced by exonuclease-deficient recombinants differed between the plasmid- and genome-based assays. The spectra of supF mutations also differed between the two assays. In addition, exonuclease-defective viruses also induced different spectra of supF and tk mutations. Therefore, both the assay methods and the target genes used for mutagenesis studies can affect the repication fidelity of herpes simplex virus type 1 Pol with defective exonuclease activity.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, NY 13210. Phone: (315) 464-8739. Fax: (315) 464-7680. E-mail: hwangc{at}mail.upstate.edu.

Journal of Virology, March 2003, p. 2946-2955, Vol. 77, No. 5
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.5.2946-2955.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tian, W., Hwang, Y. T., Lu, Q., Hwang, C. B. C. (2009). Finger Domain Mutation Affects Enzyme Activity, DNA Replication Efficiency, and Fidelity of an Exonuclease-Deficient DNA Polymerase of Herpes Simplex Virus Type 1. J. Virol. 83: 7194-7201 [Abstract] [Full Text]  
  • Drake, J. W., Bebenek, A., Kissling, G. E., Peddada, S. (2005). Clusters of mutations from transient hypermutability. Proc. Natl. Acad. Sci. USA 102: 12849-12854 [Abstract] [Full Text]  
  • Song, L., Chaudhuri, M., Knopf, C. W., Parris, D. S. (2004). Contribution of the 3'- to 5'-Exonuclease Activity of Herpes Simplex Virus Type 1 DNA Polymerase to the Fidelity of DNA Synthesis. J. Biol. Chem. 279: 18535-18543 [Abstract] [Full Text]  
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