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Journal of Virology, February 2003, p. 2675-2685, Vol. 77, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.4.2675-2685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Interaction of the Equine Herpesvirus 1 EICP0 Protein with the Immediate-Early (IE) Protein, TFIIB, and TBP May Mediate the Antagonism between the IE and EICP0 Proteins

Seong K. Kim, Hyung K. Jang,{dagger} Randy A. Albrecht, Wilbert A. Derbigny,{ddagger} Yunfei Zhang, and Dennis J. O'Callaghan*

Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130-3932

Received 17 July 2002/ Accepted 2 November 2002

The equine herpesvirus 1 (EHV-1) immediate-early (IE) and EICP0 proteins are potent trans-activators of EHV-1 promoters; however, in transient-transfection assays, the IE protein inhibits the trans-activation function of the EICP0 protein. Assays with IE mutant proteins revealed that its DNA-binding domain, TFIIB-binding domain, and nuclear localization signal may be important for the antagonism between the IE and EICP0 proteins. In vitro interaction assays with the purified IE and EICP0 proteins indicated that these proteins interact directly. At late times postinfection, the IE and EICP0 proteins colocalized in the nuclei of infected equine cells. Transient-transfection assays showed that the EICP0 protein trans-activated EHV-1 promoters harboring only a minimal promoter region (TATA box and cap site), suggesting that the EICP0 protein trans-activates EHV-1 promoters by interactions with general transcription factor(s). In vitro interaction assays revealed that the EICP0 protein interacted directly with the basal transcription factors TFIIB and TBP and that the EICP0 protein (amino acids [aa] 143 to 278) mediated the interaction with aa 125 to 174 of TFIIB. Our unpublished data showed that the IE protein interacts with the same domain (aa 125 to 174) of TFIIB and with TBP. Taken together, these results suggested that interaction of the EICP0 protein with the IE protein, TFIIB, and TBP may mediate the antagonism between the IE and EICP0 proteins.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, 1501 Kings Highway, PO Box 33932, Shreveport, LA 71130-3932. Phone: (318) 675-5750. Fax: (318) 675-5764. E-mail: docall{at}lsuhsc.edu.

{dagger} Present address: College of Veterinary Medicine, Chonbuk National University, Chonju 561-756, Korea.

{ddagger} Present address: Eli Lilly and Company, Indianapolis, IN 46285.

Journal of Virology, February 2003, p. 2675-2685, Vol. 77, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.4.2675-2685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kim, S. K., Ahn, B. C., Albrecht, R. A., O'Callaghan, D. J. (2006). The Unique IR2 Protein of Equine Herpesvirus 1 Negatively Regulates Viral Gene Expression.. J. Virol. 80: 5041-5049 [Abstract] [Full Text]  
  • Kim, S. K., Albrecht, R. A., O'Callaghan, D. J. (2004). A Negative Regulatory Element (Base Pairs -204 to -177) of the EICP0 Promoter of Equine Herpesvirus 1 Abolishes the EICP0 Protein's trans-Activation of Its Own Promoter. J. Virol. 78: 11696-11706 [Abstract] [Full Text]  


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