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Journal of Virology, February 2003, p. 2287-2294, Vol. 77, No. 3
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.3.2287-2294.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Duck Hepatitis B Virus Virion Secretion Requires a Double-Stranded DNA Genome

David Perlman and Jianming Hu*

Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts

Received 20 August 2002/ Accepted 30 October 2002

Hepatitis B virus assembly begins with the packaging of an RNA pregenome into intracellular nucleocapsids, with subsequent reverse transcription within these nucleocapsids converting the RNA into a characteristic, partially double-stranded DNA, which, alone, is found in enveloped extracellular virions as the viral genome. Using a synchronized replication system for the duck hepatitis B virus, together with a stringent two-step assay for virion secretion, we demonstrate that this selective genome secretion results from an intrinsic secretion competence gained only by the nucleocapsids at the late stage of reverse transcription.

* Corresponding author. Mailing address: Department of Microbiology, Boston University School of Medicine, 80 E. Concord St., Rm. R516, Boston, MA 02118. Phone: (617) 638-4982. Fax: (617) 638-4286. E-mail: jmhu{at}bu.edu.

Journal of Virology, February 2003, p. 2287-2294, Vol. 77, No. 3
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.3.2287-2294.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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