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Journal of Virology, February 2003, p. 2063-2070, Vol. 77, No. 3
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.3.2063-2070.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Depletion of Wee-1 Kinase Is Necessary for both Human Immunodeficiency Virus Type 1 Vpr- and Gamma Irradiation-Induced Apoptosis

Huidong Yuan, Yi-Ming Xie, and Irvin S. Y. Chen*

Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095

Received 30 July 2002/ Accepted 25 October 2002

Human immunodeficiency virus (HIV) protein R (Vpr) induces G2 arrest, and prolonged G2 arrest leads to apoptosis. We find that in HeLa cells the cell cycle regulatory kinase, Wee-1, is depleted following prolonged G2 arrest induced by Vpr. Of note, small interfering RNAs directed to Wee-1 triggered apoptosis, suggesting a direct role for Wee-1 in apoptosis. In support of this hypothesis, overexpression of Wee-1 suppressed Vpr-mediated apoptosis. Importantly, similar results were observed with cells induced to undergo apoptosis gamma irradiation. Thus, Wee-1 may serve as a key regulator of both HIV type 1 Vpr- and gamma irradiation-mediated apoptosis and possibly serve as a general regulator linking the cell cycle to some pathways of apoptosis.

* Corresponding author. Mailing address: Department of Microbiology, Immunology and Molecular Genetics, UCLA AIDS Institute, 11-934 Factor Bldg., 10833 LeConte Ave., Los Angeles, CA 90095. Phone: (310) 825-4793. Fax: (310) 267-1875. E-mail: syuchen{at}mednet.ucla.edu.

Journal of Virology, February 2003, p. 2063-2070, Vol. 77, No. 3
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.3.2063-2070.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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