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Journal of Virology, February 2003, p. 1885-1893, Vol. 77, No. 3
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.3.1885-1893.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Conditional Replication of Duck Hepatitis B Virus in Hepatoma Cells

Ju-Tao Guo,1 Melissa Pryce,1 Xingtai Wang,2 M. Inmaculada Barrasa,1 Jianming Hu,2* and Christoph Seeger1*

Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111,1 Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 021182

Received 21 June 2002/ Accepted 20 October 2002

To facilitate investigations of replication and host cell interactions in the hepadnavirus system, we have developed cell lines permitting the conditional replication of duck hepatitis B virus (DHBV). With the help of this system, we devised conditions for core particle isolation that preserve replicase activity, which was not found in previous preparations. Investigations of the stability of viral DNA intermediates indicated that both encapsidated DNA and covalently closed circular DNA (cccDNA) were turned over independently of cell division. Moreover, we showed that alpha interferon reduced the accumulation of RNA-containing viral particles. The availability of a synchronized replication system will permit the biochemical analysis of individual steps of the viral replication cycle, including the mechanism and regulation of cccDNA formation.

* Corresponding authors. Mailing address for C. Seeger: Fox Chase Cancer Center, 7701 Burholme Ave., Philadelphia, PA 19111. Phone: (215) 728-4312. Fax: (215) 728-4329. E-mail: c_seeger{at}fccc.edu. Mailing address for J. Hu: Department of Microbiology, Boston University School of Medicine, 80 E. Concord St., Boston, MA 02118. Phone: (617) 638-4982. Fax: (617) 618-4286. E-mail: jmhu{at}bu.edu.

Journal of Virology, February 2003, p. 1885-1893, Vol. 77, No. 3
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.3.1885-1893.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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