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Journal of Virology, December 2003, p. 13448-13454, Vol. 77, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.24.13448-13454.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Integrin {alpha}3ß1 Is an Alternative Cellular Receptor for Adenovirus Serotype 5

Barbara Salone,1,2 Yuri Martina,1,2 Stefania Piersanti,1,2 Enrico Cundari,3 Gioia Cherubini,1,2 Laure Franqueville,4 Cristina M. Failla,5 Pierre Boulanger,4 and Isabella Saggio1,2*

Department of Genetics and Molecular Biology, University of Rome "La Sapienza,",1 Institute of Cell Biology and Pathology, CNR,3 Laboratory of Cell Biology, IDI, Rome,5 Parco Scientifico Biomedico di Roma, San Raffaele, Italy,2 Laboratoire de Virologie et Pathogénèse Virale, CNRS UMR-5537, Faculté de Médecine Laennec, Lyon, France4

Received 16 May 2003/ Accepted 5 September 2003

Many adenovirus serotypes enter cells by high-affinity binding to the coxsackievirus-adenovirus receptor (CAR) and integrin-mediated internalization. In the present study, we analyzed the possible receptor function of {alpha}3ß1 for adenovirus serotype 5 (Ad5). We found that penton base and integrin {alpha}3ß1 could interact in vitro. In vivo, both Ad5-cell binding and virus-mediated transduction were inhibited in the presence of anti-{alpha}3 and anti-ß1 function-blocking antibodies, and this occurred in both CAR-positive and CAR-negative cell lines. Peptide library screenings and data from binding experiments with wild-type and mutant penton base proteins suggest that the Arg-Gly-Asp (RGD) in the penton base protein, the best known integrin binding motif, is only part of the binding interface with {alpha}3ß1, which involved multiple additional contact sites.

* Corresponding author. Mailing address: Dept. of Genetics and Molecular Biology, University, La Sapienza, Pzle A. Moro 5, 00185 Rome, Italy. Phone: 39 06 49912870. Fax: 39 06 4456866. E-mail: isabella.saggio{at}uniroma1.it.

Journal of Virology, December 2003, p. 13448-13454, Vol. 77, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.24.13448-13454.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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