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Journal of Virology, December 2003, p. 13425-13432, Vol. 77, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.24.13425-13432.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Proteins Associated with Murine Gammaherpesvirus 68 Virions

Eric Bortz,1 Julian P. Whitelegge,2 Qingmei Jia,3 Z. Hong Zhou,4 James P. Stewart,5 Ting-Ting Wu,3 and Ren Sun1,3*

Molecular Biology Institute,1 the Pasarow Mass Spectrometry Laboratory, Departments of Psychiatry and Biobehavioral Sciences and The Neuropsychiatric Institute,2 Department of Molecular and Medical Pharmacology, UCLA AIDS Institute, Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Los Angeles, California 90095,3 Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030,4 Department of Medical Microbiology, University of Liverpool, Liverpool L69 3GA, United Kingdom5

Received 23 May 2003/ Accepted 2 September 2003

Murine gammaherpesvirus 68 (MHV68 [also known as {gamma}HV-68]) is distinguished by its ability to replicate to high titers in cultured cells, making it an excellent candidate for studying gammaherpesvirus virion composition. Extracellular MHV68 virions were isolated, and abundant virion-associated proteins were identified by mass spectrometry. Five nucleocapsid protein homologues, the tegument protein homologue encoded by open reading frame (ORF) 75c, and envelope glycoproteins B and H were detected. In addition, gene products from MHV68 ORF20, ORF24, ORF28, ORF45, ORF48, and ORF52 were identified in association with virions, suggesting that these gammaherpesvirus genes are involved in the early phase of infection or virion assembly and egress.

* Corresponding author. Mailing address: Department of Molecular and Medical Pharmacology, University of California at Los Angeles, Los Angeles, CA 90095. Phone: (310) 794-5557. Fax: (310) 794-5123. E-mail: rsun{at}mednet.ucla.edu.

Journal of Virology, December 2003, p. 13425-13432, Vol. 77, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.24.13425-13432.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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