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Journal of Virology, December 2003, p. 13396-13398, Vol. 77, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.24.13396-13398.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Neutralization of Enteric Coronaviruses with Escherichia coli Cells Expressing Single-Chain Fv-Autotransporter Fusions

Esteban Veiga, Víctor de Lorenzo,* and Luis Angel Fernández

Department of Microbial Biotechnology, Centro Nacional de Biotecnología CSIC, Campus de Cantoblanco, 28049 Madrid, Spain

Received 6 June 2003/ Accepted 8 September 2003

We report here that fusions of single-chain antibodies (scFvs) to the autotransporter ß domain of the IgA protease of Neisseria gonorrhoeae are instrumental in locating virus-neutralizing activity on the cell surface of Escherichia coli. E. coli cells displaying scFvs against the transmissible gastroenteritis coronavirus on their surface blocked in vivo the access of the infectious agent to cultured epithelial cells. This result raises prospects for antiviral strategies aimed at hindering the entry into target cells by bacteria that naturally colonize the same intestinal niches.

* Corresponding author. Mailing address: Department of Microbial Biotechnology, Centro Nacional de Biotecnología CSIC, Campus de Cantoblanco, 28049 Madrid, Spain. Phone: 34 91 585 4536. Fax: 34 91 585 4506. E-mail: vdlorenzo{at}cnb.uam.es.

Journal of Virology, December 2003, p. 13396-13398, Vol. 77, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.24.13396-13398.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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