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Journal of Virology, December 2003, p. 12907-12913, Vol. 77, No. 23
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.23.12907-12913.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Replication-Defective Adenoviral Vaccine Vector for the Induction of Immune Responses to Dengue Virus Type 2

Smita Jaiswal, Navin Khanna, and S. Swaminathan*

International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India

Received 16 June 2003/ Accepted 1 August 2003

A recombinant replication-defective adenovirus vector that can overexpress the ectodomain of the envelope protein of dengue virus type 2 (NGC strain) has been constructed. This virus was immunogenic in mice and elicited dengue virus type 2 specific B- and T-cell responses. Sera from immunized mice contained neutralizing antibodies that could specifically recognize dengue virus type 2 and neutralize its infectivity in vitro, indicating that this approach has the potential to confer protective immunity. In vitro stimulation of splenocytes (from immunized mice) with dengue virus type 2 resulted in a significant proliferative response accompanied by the production of high levels of gamma interferon but did not show significant changes in interleukin-4 levels. This is suggestive of a Th1-like response (considered to be important in the maturation of cytotoxic T lymphocytes that are essential for the elimination of virus-infected cells). The data show that adenovirus vectors offer a promising alternative strategy for the development of dengue virus vaccines.

* Corresponding author. Mailing address: RGP Laboratory, ICGEB, P.O. Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India. Phone: 91-11-26177357, ext. 271. Fax: 91-11-26162316. E-mail: swami{at}icgeb.res.in.

Journal of Virology, December 2003, p. 12907-12913, Vol. 77, No. 23
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.23.12907-12913.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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