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Journal of Virology, December 2003, p. 12875-12880, Vol. 77, No. 23
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.23.12875-12880.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

In Vitro and In Vivo Interactions between the Hepatitis B Virus Protein P22 and the Cellular Protein gC1qR

S. Lainé,1 A. Thouard,1 J. Derancourt,2 M. Kress,3 D. Sitterlin,1 and J.-M. Rossignol1*

Laboratoire de Génétique et Biologie Cellulaire, Université de Versailles St. Quentin, Versailles,1 Centre de Recherches de Biochimie Macromoléculaire du CNRS, Montpellier,2 Institut André Lwoff, Villejuif, France3

Received 31 March 2002/ Accepted 29 August 2003

gC1qR, a mitochondrial matrix protein, was identified as the main cellular partner of the hepatitis B virus P22 protein. We demonstrated by immunofluorescence studies that some P22 molecules were colocalized with the endogenous gC1qR in both the cytoplasm and the nucleus but never in the mitochondria. We also showed that the last 34 amino acids of P22 were involved in the association with gC1qR.

* Corresponding author. Mailing address: Laboratoire de Génétique et Biologie Cellulaire, FRE 2445 CNRS-Université de Versailles St. Quentin, Versailles, France. Phone: (33) 1 39 25 45 95. Fax: (33) 1 39 25 45 72. E-mail: jean-michel.rossignol{at}genetique.uvsq.fr.

Journal of Virology, December 2003, p. 12875-12880, Vol. 77, No. 23
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.23.12875-12880.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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Copyright © 2003 by the American Society for Microbiology. All rights reserved.