This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sun, X.
Right arrow Articles by Whittaker, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sun, X.
Right arrow Articles by Whittaker, G. R.

 Previous Article  |  Next Article 

Journal of Virology, December 2003, p. 12543-12551, Vol. 77, No. 23
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.23.12543-12551.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role for Influenza Virus Envelope Cholesterol in Virus Entry and Infection

Xiangjie Sun and Gary R. Whittaker*

Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853

Received 28 April 2003/ Accepted 22 August 2003

Enveloped viruses are highly dependent on their lipid envelopes for entry into and infection of host cells. Here, we have examined the role of cholesterol in the virus envelope, using methyl-ß-cyclodextrin depletion. Pretreatment of virions with methyl-ß-cyclodextrin efficiently depleted envelope cholesterol from influenza virus and significantly reduced virus infectivity in a dose-dependent manner. A nonenveloped virus, simian virus 40, was not affected by methyl-ß-cyclodextrin treatment. In the case of influenza virus, infectivity could be partially rescued by the addition of exogenous cholesterol. Influenza virus morphology, binding, and internalization were not affected by methyl-ß-cyclodextrin depletion, whereas envelope cholesterol depletion markedly affected influenza virus fusion, as measured by a specific reduction in the infectivity of viruses induced to fuse at the cell surface and by fluorescence-dequenching assays. These data suggest that envelope cholesterol is a critical factor in the fusion process of influenza virus.

* Corresponding author. Mailing address: C4 127 Veterinary Medical Center, Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853. Phone: (607) 253-4019. Fax: (607) 253-3384. E-mail: grw7{at}cornell.edu.

Journal of Virology, December 2003, p. 12543-12551, Vol. 77, No. 23
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.23.12543-12551.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Clemente, R., de Parseval, A., Perez, M., de la Torre, J. C. (2009). Borna Disease Virus Requires Cholesterol in both Cellular Membrane and Viral Envelope for Efficient Cell Entry. J. Virol. 83: 2655-2662 [Abstract] [Full Text]  
  • Lee, C.-J., Lin, H.-R., Liao, C.-L., Lin, Y.-L. (2008). Cholesterol Effectively Blocks Entry of Flavivirus. J. Virol. 82: 6470-6480 [Abstract] [Full Text]  
  • Biswas, S., Yin, S.-R., Blank, P. S., Zimmerberg, J. (2008). Cholesterol Promotes Hemifusion and Pore Widening in Membrane Fusion Induced by Influenza Hemagglutinin. JGP 131: 503-513 [Abstract] [Full Text]  
  • Barman, S., Nayak, D. P. (2007). Lipid Raft Disruption by Cholesterol Depletion Enhances Influenza A Virus Budding from MDCK Cells. J. Virol. 81: 12169-12178 [Abstract] [Full Text]  
  • Raghu, H., Sharma-Walia, N., Veettil, M. V., Sadagopan, S., Caballero, A., Sivakumar, R., Varga, L., Bottero, V., Chandran, B. (2007). Lipid Rafts of Primary Endothelial Cells Are Essential for Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8-Induced Phosphatidylinositol 3-Kinase and RhoA-GTPases Critical for Microtubule Dynamics and Nuclear Delivery of Viral DNA but Dispensable for Binding and Entry. J. Virol. 81: 7941-7959 [Abstract] [Full Text]  
  • Imhoff, H., von Messling, V., Herrler, G., Haas, L. (2007). Canine Distemper Virus Infection Requires Cholesterol in the Viral Envelope. J. Virol. 81: 4158-4165 [Abstract] [Full Text]  
  • Kapadia, S. B., Barth, H., Baumert, T., McKeating, J. A., Chisari, F. V. (2007). Initiation of Hepatitis C Virus Infection Is Dependent on Cholesterol and Cooperativity between CD81 and Scavenger Receptor B Type I. J. Virol. 81: 374-383 [Abstract] [Full Text]  
  • Bukrinsky, M., Sviridov, D. (2006). Human immunodeficiency virus infection and macrophage cholesterol metabolism.. J. Leukoc. Biol. 80: 1044-1051 [Abstract] [Full Text]  
  • Marjuki, H., Alam, M. I., Ehrhardt, C., Wagner, R., Planz, O., Klenk, H.-D., Ludwig, S., Pleschka, S. (2006). Membrane Accumulation of Influenza A Virus Hemagglutinin Triggers Nuclear Export of the Viral Genome via Protein Kinase C{alpha}-mediated Activation of ERK Signaling. J. Biol. Chem. 281: 16707-16715 [Abstract] [Full Text]  
  • Huang, H., Li, Y., Sadaoka, T., Tang, H., Yamamoto, T., Yamanishi, K., Mori, Y. (2006). Human herpesvirus 6 envelope cholesterol is required for virus entry. J. Gen. Virol. 87: 277-285 [Abstract] [Full Text]  
  • Hess, S. T., Kumar, M., Verma, A., Farrington, J., Kenworthy, A., Zimmerberg, J. (2005). Quantitative electron microscopy and fluorescence spectroscopy of the membrane distribution of influenza hemagglutinin. JCB 169: 965-976 [Abstract] [Full Text]  
  • Chung, C.-S., Huang, C.-Y., Chang, W. (2005). Vaccinia Virus Penetration Requires Cholesterol and Results in Specific Viral Envelope Proteins Associated with Lipid Rafts. J. Virol. 79: 1623-1634 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»