This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hirashima, K. Articles by Watanabe, Y. Search for Related Content PubMed PubMed Citation Articles by Hirashima, K. Articles by Watanabe, Y.

 Previous Article  |  Next Article 

Journal of Virology, November 2003, p. 12357-12362, Vol. 77, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.22.12357-12362.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

RNA Helicase Domain of Tobamovirus Replicase Executes Cell-to-Cell Movement Possibly through Collaboration with Its Nonconserved Region

Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902,¹ R&D Group, ASPEX Division, Asahi Glass Co., Ltd., Kanagawa-ku, Yokohama-shi, Kanagawa 221-8755, Japan²

Received 19 May 2003/ Accepted 1 August 2003

UR-hel, a chimeric virus obtained by replacement of the RNA helicase domain of tobacco mosaic virus (TMV)-U1 replicase with that from the TMV-R strain, could replicate similarly to TMV-U1 in protoplasts but could not move from cell to cell (K. Hirashima and Y. Watanabe, J. Virol. 75:8831-8836, 2001). It was suggested that TMV recruited both the movement protein (MP) and replicase for cell-to-cell movement by unknown mechanisms. Here, we found that a recombinant, UR-hel/V, in which the nonconserved region was derived from TMV-R in addition to the RNA helicase domain of replicase, could move from cell to cell. We also analyzed revertants isolated from UR-hel, which recovered cell-to-cell movement by their own abilities. We found amino acid substitutions responsible for phenotypic reversion only in the nonconserved region and/or RNA helicase domain but never in MP. Together, these data show that both the nonconserved region and the RNA helicase domain of replicase are involved in cell-to-cell movement. The RNA helicase domain of tobamovirus replicase possibly does not interact directly with MP but interacts with its nonconserved region to execute cell-to-cell movement.

This article has been cited by other articles:

• Ashby, J., Boutant, E., Seemanpillai, M., Sambade, A., Ritzenthaler, C., Heinlein, M. (2006). Tobacco mosaic virus movement protein functions as a structural microtubule-associated protein.. J. Virol. 80: 8329-8344 [Abstract] [Full Text]  
• Hwang, M. S., Kim, S. H., Lee, J. H., Bae, J. M., Paek, K. H., Park, Y. I. (2005). Evidence for interaction between the 2a polymerase protein and the 3a movement protein of Cucumber mosaic virus. J. Gen. Virol. 86: 3171-3177 [Abstract] [Full Text]  
• Boevink, P., Oparka, K. J. (2005). Virus-Host Interactions during Movement Processes. Plant Physiol. 138: 1815-1821 [Full Text]  
• Liu, J.-Z., Blancaflor, E. B., Nelson, R. S. (2005). The Tobacco Mosaic Virus 126-Kilodalton Protein, a Constituent of the Virus Replication Complex, Alone or within the Complex Aligns with and Traffics along Microfilaments. Plant Physiol. 138: 1853-1865 [Abstract] [Full Text]  
• Choi, S. K., Palukaitis, P., Min, B. E., Lee, M. Y., Choi, J. K., Ryu, K. H. (2005). Cucumber mosaic virus 2a polymerase and 3a movement proteins independently affect both virus movement and the timing of symptom development in zucchini squash. J. Gen. Virol. 86: 1213-1222 [Abstract] [Full Text]