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Journal of Virology, November 2003, p. 12352-12356, Vol. 77, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.22.12352-12356.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Lymphatic Mechanism of Rotavirus Extraintestinal Spread in the Neonatal Mouse

Eric C. Mossel{dagger} and Robert F. Ramig*

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030

Received 10 February 2003/ Accepted 18 August 2003

We used the neonatal mouse model of rotavirus infection and virus strains SA11-clone 4 (SA11-Cl4) and Rhesus rotavirus (RRV) to examine the mechanism of the extraintestinal spread of viruses following oral inoculation. The spread-competent viruses, RRV and reassortant R7, demonstrated a temporal progression from the intestine, to the terminal ileum, to the mesenteric lymph nodes (MLN), and to the peripheral tissues. SA11-Cl4 was not found outside the intestine. Reassortant virus S7, which was unable to reach the liver in previous studies (E. C. Mossel and R. F. Ramig, J. Virol. 76:6502-6509, 2002), was recovered from 60% of the MLN, suggesting that there are multiple determinants for the spread of virus from the intestine to the MLN. Phenotypic segregation analysis identified RRV genome segment 6 (VP6) as a secondary determinant of the spread of virus to the MLN (P = 0.02) in reassortant viruses containing segment 7 from the spread-incompetent parent. These data suggest that in the orally infected neonatal mouse, the extraintestinal spread of rotavirus occurs via a lymphatic pathway, and the spread phenotype is primarily determined by NSP3 and can be modified by VP6.

* Corresponding author. Mailing address: Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030. Phone: (713) 798-4830. Fax: (713) 798-5025. E-mail: rramig{at}bcm.tmc.edu.

{dagger} Present address: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77030.

Journal of Virology, November 2003, p. 12352-12356, Vol. 77, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.22.12352-12356.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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