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Journal of Virology, November 2003, p. 12346-12351, Vol. 77, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.22.12346-12351.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

High Frequency of Virus-Specific CD8+ T Cells in the Central Nervous System of Macaques Chronically Infected with Simian Immunodeficiency Virus SIVmac251

Marcin Moniuszko,1 Charlie Brown,2 Ranajit Pal,3 Elzbieta Tryniszewska ,1,{dagger} Wen-Po Tsai,1 Vanessa M. Hirsch,2 and Genoveffa Franchini1*

Animal Models and Retroviral Vaccines Section, National Cancer Institute, Bethesda, Maryland 20892-5055,1 Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Disease, Rockville, Maryland 20852,2 and Advanced BioScience Laboratories, Inc., Kensington, Maryland 208953

Received 24 March 2003/ Accepted 7 August 2003

Infection with human immunodeficiency virus or simian immunodeficiency virus (SIV) induces virus-specific CD8+ T cells that traffic to lymphoid and nonlymphoid tissues. In this study, we used Gag-specific tetramer staining to investigate the frequency of CD8+ T cells in peripheral blood and the central nervous system of Mamu-A*01-positive SIV-infected rhesus macaques. Most of these infected macaques were vaccinated prior to SIVmac251 exposure. The frequency of Gag181-189 CM9 tetramer-positive cells was consistently higher in the cerebrospinal fluid and the brain than in the blood of all animals studied and did not correlate with either plasma viremia or CD4+-T-cell level. Little or no infection in the brain was documented for most animals by nucleic acid sequence-based amplification or in situ hybridization. These data suggest that this Gag-specific response may contribute to the containment of viral replication in this locale.

* Corresponding author. Mailing address: Animal Models and Retroviral Vaccines Section, NCI, 41/D804, Bethesda, MD 20892-5055. Phone: (301) 496-2386. Fax: (301) 402-0055. E-mail: gf7u{at}nih.gov.

{dagger} Present address: Third Department of Pediatrics, Medical Academy of Bialystok, Waszyngtona 17, Poland.

Journal of Virology, November 2003, p. 12346-12351, Vol. 77, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.22.12346-12351.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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