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Journal of Virology, November 2003, p. 11910-11917, Vol. 77, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.22.11910-11917.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Resistance of Human Hepatitis Delta Virus RNAs to Dicer Activity

Jinhong Chang,1 Patrick Provost,2 and John M. Taylor1*

Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111-2497,1 Laval University, Ste Foy, Quebec G1V 4G2, Canada2

Received 22 May 2003/ Accepted 18 August 2003

The endonuclease dicer cleaves RNAs that are 100% double stranded and certain RNAs with extensive but <100% pairing to release ~21-nucleotide (nt) fragments. Circular 1,679-nt genomic and antigenomic RNAs of human hepatitis delta virus (HDV) can fold into a rod-like structure with 74% pairing. However, during HDV replication in hepatocytes of human, woodchuck, and mouse origin, no ~21-nt RNAs were detected. Likewise, in vitro, purified recombinant dicer gave <0.2% cleavage of unit-length HDV RNAs. Similarly, rod-like RNAs of potato spindle tuber viroid (PSTVd) and avocado sunblotch viroid (ASBVd) were only 0.5% cleaved. Furthermore, when a 66-nt hairpin RNA with 79% pairing, the putative precursor to miR-122, which is an abundant liver micro-RNA, replaced one end of HDV genomic RNA, it was poorly cleaved, both in vivo and in vitro. In contrast, this 66-nt hairpin, in the absence of appended HDV sequences, was >80% cleaved in vitro. Other 66-nt hairpins derived from one end of genomic HDV, PSTVd, or ASBVd RNAs were also cleaved. Apparently, for unit-length RNAs of HDV, PSTVd, and ASBVd, it is the extended structure with <100% base pairing that confers significant resistance to dicer action.

* Corresponding author. Mailing address: Fox Chase Cancer Center, Philadelphia, PA 19111-2497. Phone: (215) 728-2436. Fax: (215) 728-3105. E-mail: JM_Taylor{at}fccc.edu.

Journal of Virology, November 2003, p. 11910-11917, Vol. 77, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.22.11910-11917.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chang, J., Guo, J.-T., Jiang, D., Guo, H., Taylor, J. M., Block, T. M. (2008). Liver-Specific MicroRNA miR-122 Enhances the Replication of Hepatitis C Virus in Nonhepatic Cells. J. Virol. 82: 8215-8223 [Abstract] [Full Text]  
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