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Journal of Virology, November 2003, p. 11846-11848, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11846-11848.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Binding of Transmissible Gastroenteritis Coronavirus to Brush Border Membrane Sialoglycoproteins

Christel Schwegmann-Wessels,1* Gert Zimmer,1 Bernd Schröder,2 Gerhard Breves,2 and Georg Herrler1

Institut für Virologie,1 Physiologisches Institut, Tierärztliche Hochschule Hannover, 30559 Hannover, Germany2

Received 2 May 2003/ Accepted 30 July 2003

Transmissible gastroenteritis coronavirus (TGEV) is a porcine pathogen causing enteric infections that are lethal for suckling piglets. The enterotropism of TGEV is connected with the sialic acid binding activity of the viral surface protein S. Here we show that, among porcine intestinal brush border membrane proteins, TGEV recognizes a mucin-type glycoprotein designated MGP in a sialic acid-dependent fashion. Virus binding assays with cryosections of the small intestine from a suckling piglet revealed the binding of TGEV to mucin-producing goblet cells. A nonenteropathogenic mutant virus that lacked a sialic acid binding activity was unable to bind to MGP and to attach to goblet cells. Our results suggest a role of MGP in the enteropathogenicity of TGEV.

* Corresponding author. Mailing address: Institut für Virologie, Tierärztliche Hochschule Hannover, Bünteweg 17, 30559 Hannover, Germany. Phone: 49 (0) 511-28-8842. Fax: 49 (0) 511-28-8898. E-mail: Christel.Schwegmann{at}tiho-hannover.de.

Journal of Virology, November 2003, p. 11846-11848, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11846-11848.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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