This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schneider, U.
Right arrow Articles by Schwemmle, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schneider, U.
Right arrow Articles by Schwemmle, M.

 Previous Article  |  Next Article 

Journal of Virology, November 2003, p. 11781-11789, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11781-11789.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Active Borna Disease Virus Polymerase Complex Requires a Distinct Nucleoprotein-to-Phosphoprotein Ratio but No Viral X Protein

Urs Schneider,* Melanie Naegele, Peter Staeheli,* and Martin Schwemmle{dagger}

Department of Virology, University of Freiburg, D-79104 Freiburg, Germany

Received 2 May 2003/ Accepted 6 August 2003

Analysis of the composition and regulation of the Borna disease virus (BDV) polymerase complex has so far been limited by the lack of a functional assay. To establish such an assay on the basis of an artificial minigenome, we constructed expression vectors encoding either nucleoprotein (N), phosphoprotein (P), X protein, or polymerase (L) of BDV under the control of the chicken ß-actin promoter. A Flag-tagged version of L colocalized with virus-encoded N and P in characteristic nuclear dots of BDV-infected cells and increased viral N-protein levels in persistently infected Vero cells. Vector-driven expression of L, N, and P in BSR-T7 cells together with a negative-sense BDV minigenome carrying a chloramphenicol acetyltransferase (CAT) reporter gene resulted in efficient synthesis of CAT protein. Induction of CAT protein synthesis strongly depended on a 10- to 30-fold molar excess of the N-encoding plasmid over the P-encoding plasmid. Cotransfection of even small amounts of plasmid encoding the viral X protein reduced CAT synthesis to background levels. Thus, the N-to-P stoichiometry seems to play a central role in the regulation of the BDV polymerase complex. Our data further suggest a negative regulatory function for the X protein of BDV.

* Corresponding author. Mailing address for Peter Staeheli and Urs Schneider: Department of Virology, University of Freiburg, Hermann-Herder-Strasse 11, D-79104 Freiburg, Germany. Phone: 49-761-203-6579. Fax: 49-761-203-5350. E-mail for Peter Staeheli: staeheli{at}ukl.uni-freiburg.de. Phone for Urs Schneider: 49-761-203-6616. E-mail: ursschn{at}ukl.uni-freiburg.de.

{dagger} Present address: Institute of Medical Virology, University of Zürich, Zürich, Switzerland.

Journal of Virology, November 2003, p. 11781-11789, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11781-11789.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Poenisch, M., Wille, S., Schneider, U., Staeheli, P. (2009). Second-site mutations in Borna disease virus overexpressing viral accessory protein X. J. Gen. Virol. 90: 1932-1936 [Abstract] [Full Text]  
  • Poenisch, M., Burger, N., Staeheli, P., Bauer, G., Schneider, U. (2009). Protein X of Borna Disease Virus Inhibits Apoptosis and Promotes Viral Persistence in the Central Nervous Systems of Newborn-Infected Rats. J. Virol. 83: 4297-4307 [Abstract] [Full Text]  
  • Poenisch, M., Wille, S., Staeheli, P., Schneider, U. (2008). Polymerase Read-Through at the First Transcription Termination Site Contributes to Regulation of Borna Disease Virus Gene Expression. J. Virol. 82: 9537-9545 [Abstract] [Full Text]  
  • Poenisch, M., Staeheli, P., Schneider, U. (2008). Viral accessory protein X stimulates the assembly of functional Borna disease virus polymerase complexes. J. Gen. Virol. 89: 1442-1445 [Abstract] [Full Text]  
  • Ackermann, A., Kugel, D., Schneider, U., Staeheli, P. (2007). Enhanced polymerase activity confers replication competence of Borna disease virus in mice. J. Gen. Virol. 88: 3130-3132 [Abstract] [Full Text]  
  • Ackermann, A., Staeheli, P., Schneider, U. (2007). Adaptation of Borna Disease Virus to New Host Species Attributed to Altered Regulation of Viral Polymerase Activity. J. Virol. 81: 7933-7940 [Abstract] [Full Text]  
  • Poenisch, M., Wille, S., Ackermann, A., Staeheli, P., Schneider, U. (2007). The X Protein of Borna Disease Virus Serves Essential Functions in the Viral Multiplication Cycle. J. Virol. 81: 7297-7299 [Abstract] [Full Text]  
  • Schneider, U., Ackermann, A., Staeheli, P. (2007). A Borna Disease Virus Vector for Expression of Foreign Genes in Neurons of Rodents. J. Virol. 81: 7293-7296 [Abstract] [Full Text]  
  • Schmid, S., Mayer, D., Schneider, U., Schwemmle, M. (2007). Functional Characterization of the Major and Minor Phosphorylation Sites of the P Protein of Borna Disease Virus. J. Virol. 81: 5497-5507 [Abstract] [Full Text]  
  • Chase, G., Mayer, D., Hildebrand, A., Frank, R., Hayashi, Y., Tomonaga, K., Schwemmle, M. (2007). Borna Disease Virus Matrix Protein Is an Integral Component of the Viral Ribonucleoprotein Complex That Does Not Interfere with Polymerase Activity. J. Virol. 81: 743-749 [Abstract] [Full Text]  
  • Martin, A., Staeheli, P., Schneider, U. (2006). RNA Polymerase II-Controlled Expression of Antigenomic RNA Enhances the Rescue Efficacies of Two Different Members of the Mononegavirales Independently of the Site of Viral Genome Replication.. J. Virol. 80: 5708-5715 [Abstract] [Full Text]  
  • Yanai, H., Kobayashi, T., Hayashi, Y., Watanabe, Y., Ohtaki, N., Zhang, G., de la Torre, J. C., Ikuta, K., Tomonaga, K. (2006). A Methionine-Rich Domain Mediates CRM1-Dependent Nuclear Export Activity of Borna Disease Virus Phosphoprotein. J. Virol. 80: 1121-1129 [Abstract] [Full Text]  
  • Schwardt, M., Mayer, D., Frank, R., Schneider, U., Eickmann, M., Planz, O., Wolff, T., Schwemmle, M. (2005). The negative regulator of Borna disease virus polymerase is a non-structural protein. J. Gen. Virol. 86: 3163-3169 [Abstract] [Full Text]  
  • Mayer, D., Fischer, H., Schneider, U., Heimrich, B., Schwemmle, M. (2005). Borna Disease Virus Replication in Organotypic Hippocampal Slice Cultures from Rats Results in Selective Damage of Dentate Granule Cells. J. Virol. 79: 11716-11723 [Abstract] [Full Text]  
  • Perez, M., de la Torre, J. C. (2005). Identification of the Borna disease virus (BDV) proteins required for the formation of BDV-like particles. J. Gen. Virol. 86: 1891-1895 [Abstract] [Full Text]  
  • Rosario, D., Perez, M., de la Torre, J. C. (2005). Functional Characterization of the Genomic Promoter of Borna Disease Virus (BDV): Implications of 3'-Terminal Sequence Heterogeneity for BDV Persistence. J. Virol. 79: 6544-6550 [Abstract] [Full Text]  
  • Volmer, R., Bajramovic, J. J., Schneider, U., Ufano, S., Pochet, S., Gonzalez-Dunia, D. (2005). Mechanism of the Antiviral Action of 1-{beta}-D-Arabinofuranosylcytosine on Borna Disease Virus. J. Virol. 79: 4514-4518 [Abstract] [Full Text]  
  • Schneider, U., Schwemmle, M., Staeheli, P. (2005). Genome trimming: A unique strategy for replication control employed by Borna disease virus. Proc. Natl. Acad. Sci. USA 102: 3441-3446 [Abstract] [Full Text]  
  • Schneider, U., Blechschmidt, K., Schwemmle, M., Staeheli, P. (2004). Overlap of Interaction Domains Indicates a Central Role of the P Protein in Assembly and Regulation of the Borna Disease Virus Polymerase Complex. J. Biol. Chem. 279: 55290-55296 [Abstract] [Full Text]  
  • Poenisch, M., Unterstab, G., Wolff, T., Staeheli, P., Schneider, U. (2004). The X protein of Borna disease virus regulates viral polymerase activity through interaction with the P protein. J. Gen. Virol. 85: 1895-1898 [Abstract] [Full Text]  
  • Rauer, M., Gotz, J., Schuppli, D., Staeheli, P., Hausmann, J. (2004). Transgenic Mice Expressing the Nucleoprotein of Borna Disease Virus in either Neurons or Astrocytes: Decreased Susceptibility to Homotypic Infection and Disease. J. Virol. 78: 3621-3632 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»