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Journal of Virology, November 2003, p. 11625-11632, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11625-11632.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of a Human Papillomavirus Type 16-Specific Epitope on the C-Terminal Arm of the Major Capsid Protein L1

Joseph J. Carter,1* Greg C. Wipf,1 Sarah F. Benki,1 Neil D. Christensen,2 and Denise A. Galloway1,3

Program in Cancer Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024,1 Departments of Pathology and of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033,2 Department of Microbiology, University of Washington, Seattle, Washington 98195-72423

Received 9 June 2003/ Accepted 23 July 2003

To characterize epitopes on human papillomavirus (HPV) virus-like particles (VLPs), a panel of mutated HPV-16 VLPs was created. Each mutated VLP had residues substituted from HPV-31 or HPV-52 L1 sequences to the HPV-16 L1 backbone. Mutations were created on the HPV-31 and -52 L1 proteins to determine if HPV-16 type-specific recognition could be transferred. Correct folding of the mutated proteins was verified by resistance to trypsin digestion and by binding to one or more conformation-dependent monoclonal antibodies. Several of the antibodies tested were found to bind to regions already identified as being important for HPV VLP recognition (loops DE, EF, FG, and HI). Sequences at both ends of the long FG loop (amino acids 260 to 290) were required for both H16.V5 and H16.E70 reactivity. A new antibody-binding site was discovered on the C-terminal arm of L1 between positions 427 and 445. Recognition of these residues by the H16.U4 antibody suggests that this region is surface exposed and supports a recently proposed molecular model of HPV VLPs.

* Corresponding author. Mailing address: Program in Cancer Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024. Phone: (206) 667-4507. Fax: (206) 667-5815. E-mail: jcarter{at}fhcrc.org.

Journal of Virology, November 2003, p. 11625-11632, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11625-11632.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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