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Journal of Virology, November 2003, p. 11491-11498, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11491-11498.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Formation of Polyomavirus-Like Particles with Different VP1 Molecules That Bind the Urokinase Plasminogen Activator Receptor

Young C. Shin{dagger} and William R. Folk*

Department of Biochemistry, University of Missouri-Columbia, Columbia, Missouri 65211

Received 9 June 2003/ Accepted 6 August 2003

Icosahedral virus-like particles formed by the self-assembly of polyomavirus capsid proteins (Py-VLPs) can serve as useful nanostructures for delivering nucleic acids, proteins, and pharmaceuticals into animal cells and tissues. Four predominant surface-exposed loops in the VP1 structure offer potential sites to display sequences that might contribute new targeting specificities. Introduction into each of these loops of sequences derived from the amino-terminal fragment of urokinase plasminogen activator (uPA) or a related phage display peptide reduced the solubility of VP1 molecules when expressed in insect cells, and insertions into the EF loop reduced VP1 solubility least. Coexpression in insect cells of the uPA-VP1 molecules and VP1 containing a FLAG epitope in the HI loop permitted the formation of heterotypic Py-VLPs containing uPA-VP1 and FLAG-VP1. These heterotypic VLPs bound to uPAR on the surfaces of animal cells. Heterotypic Py-VLPs containing ligands for multiple cell surface receptors should be useful for targeting specific cells and tissues.

* Corresponding author. Mailing address: Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211. Phone: (573) 882-2841. Fax: (573) 884-4808. E-mail: folkw{at}missouri.edu.

{dagger} Present address: Tumor Virology Division, New England Regional Primate Research Center, Southborough, Mass.

Journal of Virology, November 2003, p. 11491-11498, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11491-11498.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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