This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roy, J.
Right arrow Articles by Rethwilm, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roy, J.
Right arrow Articles by Rethwilm, A.

 Previous Article  |  Next Article 

Journal of Virology, November 2003, p. 11324-11331, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11324-11331.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Feline Foamy Virus Genome and Replication Strategy

Jacqueline Roy,1 Wolfram Rudolph,1 Thomas Juretzek,1 Kathleen Gärtner,1 Michael Bock,2,{dagger} Ottmar Herchenröder,1 Dirk Lindemann,1,2 Martin Heinkelein,1,2 and Axel Rethwilm1,2*

Institut für Virologie, Medizinische Fakultät "Carl Gustav Carus," Technische Universität Dresden, Dresden,1 Institut für Virologie und Immunbiologie, Universität Würzburg, Würzburg, Germany2

Received 28 March 2003/ Accepted 23 July 2003

Crucial aspects of the foamy virus (FV) replication strategy have so far only been investigated for the prototypic FV (PFV) isolate, which is supposed to be derived from nonhuman primates. To study whether the unusual features of this replication pathway also apply to more-distantly related FVs, we constructed feline FV (FFV) infectious molecular clones and vectors. It is shown by quantitative RNA and DNA PCR analysis that FFV virions contain more RNA than DNA. Full-length linear DNA was found in extracellular FFV by Southern blot analysis. Similar to PFV, azidothymidine inhibition experiments and the transfection of nucleic acids extracted from extracellular FFV indicated that DNA is the functional relevant FFV genome. Unlike PFV, no evidence was found indicating that FFV recycles its DNA into the nucleus.

* Corresponding author. Mailing address: Institut für Virologie und Immunbiologie, Versbacher Str. 7, 97078 Würzburg, Germany. Phone: 49-931-201-49554. Fax: 49-931-201-49553. E-mail: virologie{at}mail.uni-wuerzburg.de.

{dagger} Present address: Division of Virology, National Institute for Medical Research, London, United Kingdom.

Journal of Virology, November 2003, p. 11324-11331, Vol. 77, No. 21
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.21.11324-11331.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lee, E.-G., Linial, M. L. (2008). The C Terminus of Foamy Retrovirus Gag Contains Determinants for Encapsidation of Pol Protein into Virions. J. Virol. 82: 10803-10810 [Abstract] [Full Text]  
  • Yap, M. W., Lindemann, D., Stanke, N., Reh, J., Westphal, D., Hanenberg, H., Ohkura, S., Stoye, J. P. (2008). Restriction of Foamy Viruses by Primate Trim5{alpha}. J. Virol. 82: 5429-5439 [Abstract] [Full Text]  
  • Roy, J., Linial, M. L. (2007). Role of the Foamy Virus Pol Cleavage Site in Viral Replication. J. Virol. 81: 4956-4962 [Abstract] [Full Text]  
  • Mannigel, I., Stange, A., Zentgraf, H., Lindemann, D. (2007). Correct Capsid Assembly Mediated by a Conserved YXXLGL Motif in Prototype Foamy Virus Gag Is Essential for Infectivity and Reverse Transcription of the Viral Genome. J. Virol. 81: 3317-3326 [Abstract] [Full Text]  
  • Cartellieri, M., Herchenroder, O., Rudolph, W., Heinkelein, M., Lindemann, D., Zentgraf, H., Rethwilm, A. (2005). N-Terminal Gag Domain Required for Foamy Virus Particle Assembly and Export. J. Virol. 79: 12464-12476 [Abstract] [Full Text]  
  • Peters, K., Wiktorowicz, T., Heinkelein, M., Rethwilm, A. (2005). RNA and Protein Requirements for Incorporation of the Pol Protein into Foamy Virus Particles. J. Virol. 79: 7005-7013 [Abstract] [Full Text]  
  • Juretzek, T., Holm, T., Gartner, K., Kanzler, S., Lindemann, D., Herchenroder, O., Picard-Maureau, M., Rammling, M., Heinkelein, M., Rethwilm, A. (2004). Foamy Virus Integration. J. Virol. 78: 2472-2477 [Abstract] [Full Text]  
  • Heinkelein, M., Rammling, M., Juretzek, T., Lindemann, D., Rethwilm, A. (2003). Retrotransposition and Cell-to-Cell Transfer of Foamy Viruses. J. Virol. 77: 11855-11858 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»