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Journal of Virology, October 2003, p. 11290-11295, Vol. 77, No. 20
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.20.11290-11295.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Recovery of Infectious Foot-and-Mouth Disease Virus from Suckling Mice after Direct Inoculation with In Vitro-Transcribed RNA

Eric Baranowski,1,2,{dagger} Nicolás Molina,1,{ddagger} José Ignacio Núñez,1,2 Francisco Sobrino,1,2 and Margarita Sáiz2*

Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, 28049 Madrid,1 Centro de Investigación en Sanidad Animal, INIA, 28130 Valdeolmos, Madrid, Spain2

Received 23 April 2003/ Accepted 15 July 2003

We assayed the infectivity of naked foot-and-mouth disease virus (FMDV) RNA by direct inoculation of suckling mice. Our results demonstrate that transcripts generated from full-length cDNA clones were infectious, as was virion-extracted RNA. Interestingly, infectious virus could be recovered from a mutant transcript encoding amino acid substitution L-147->P in capsid protein VP1, known to be noninfectious for BHK-21 cells. The model described here provides a useful tool for virulence studies in vivo, bypassing possible selection of variants during viral replication in cell culture.

* Corresponding author. Mailing address: Centro de Investigación en Sanidad Animal, INIA, 28130 Valdeolmos, Madrid, Spain. Phone: 34-91-6202300. Fax: 34-91-6202247. E-mail: saiz{at}inia.es.

{dagger} Present address: Institut National de la Recherche Agronomique (INRA), UMR 1225, Ecole Nationale Vétérinaire, 31076 Toulouse Cedex 3, France.

{ddagger} Permanent address: Department of Biology, Universidad del Atlántico, Barranquilla, Colombia.

Journal of Virology, October 2003, p. 11290-11295, Vol. 77, No. 20
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.20.11290-11295.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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