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Journal of Virology, October 2003, p. 11290-11295, Vol. 77, No. 20
0022-538X/03/$08.00+0 DOI: 10.1128/JVI.77.20.11290-11295.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Recovery of Infectious Foot-and-Mouth Disease Virus from Suckling Mice after Direct Inoculation with In Vitro-Transcribed RNA
Eric Baranowski,1,2,
Nicolás Molina,1,
José Ignacio Núñez,1,2 Francisco Sobrino,1,2 and Margarita Sáiz2*
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, 28049 Madrid,1
Centro de Investigación en Sanidad Animal, INIA, 28130 Valdeolmos, Madrid, Spain2
Received 23 April 2003/
Accepted 15 July 2003
We assayed the infectivity of naked foot-and-mouth disease virus (FMDV) RNA by direct inoculation of suckling mice. Our results demonstrate that transcripts generated from full-length cDNA clones were infectious, as was virion-extracted RNA. Interestingly, infectious virus could be recovered from a mutant transcript encoding amino acid substitution L-147
P in capsid protein VP1, known to be noninfectious for BHK-21 cells. The model described here provides a useful tool for virulence studies in vivo, bypassing possible selection of variants during viral replication in cell culture.
* Corresponding author. Mailing address: Centro de Investigación en Sanidad Animal, INIA, 28130 Valdeolmos, Madrid, Spain. Phone: 34-91-6202300. Fax: 34-91-6202247. E-mail:
saiz{at}inia.es.
Present address: Institut National de la Recherche Agronomique (INRA), UMR 1225, Ecole Nationale Vétérinaire, 31076 Toulouse Cedex 3, France.
Permanent address: Department of Biology, Universidad del Atlántico, Barranquilla, Colombia.
Journal of Virology, October 2003, p. 11290-11295, Vol. 77, No. 20
0022-538X/03/$08.00+0 DOI: 10.1128/JVI.77.20.11290-11295.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
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