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Recovery of Infectious Foot-and-Mouth Disease Virus from Suckling Mice after Direct Inoculation with In Vitro-Transcribed RNA

Eric Baranowski,^1,2, Nicolás Molina,^1, José Ignacio Núñez,^1,2 Francisco Sobrino,^1,2 and Margarita Sáiz^2*

Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, 28049 Madrid,¹ Centro de Investigación en Sanidad Animal, INIA, 28130 Valdeolmos, Madrid, Spain²

Received 23 April 2003/ Accepted 15 July 2003

We assayed the infectivity of naked foot-and-mouth disease virus (FMDV) RNA by direct inoculation of suckling mice. Our results demonstrate that transcripts generated from full-length cDNA clones were infectious, as was virion-extracted RNA. Interestingly, infectious virus could be recovered from a mutant transcript encoding amino acid substitution L-147P in capsid protein VP1, known to be noninfectious for BHK-21 cells. The model described here provides a useful tool for virulence studies in vivo, bypassing possible selection of variants during viral replication in cell culture.

Present address: Institut National de la Recherche Agronomique (INRA), UMR 1225, Ecole Nationale Vétérinaire, 31076 Toulouse Cedex 3, France.

Permanent address: Department of Biology, Universidad del Atlántico, Barranquilla, Colombia.

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