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Journal of Virology, October 2003, p. 11284-11289, Vol. 77, No. 20
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.20.11284-11289.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The 5' Untranslated Region of Alfalfa Mosaic Virus RNA 1 Is Involved in Negative-Strand RNA Synthesis

A. Corina Vlot and John F. Bol*

Institute of Biology, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands

Received 25 April 2003/ Accepted 15 July 2003

The three genomic RNAs of alfalfa mosaic virus each contain a unique 5' untranslated region (5' UTR). Replacement of the 5' UTR of RNA 1 by that of RNA 2 or 3 yielded infectious replicons. The sequence of a putative 5' stem-loop structure in RNA 1 was found to be required for negative-strand RNA synthesis. A similar putative 5' stem-loop structure is present in RNA 2 but not in RNA 3.

* Corresponding author. Mailing address: Institute of Biology, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands. Phone: (31) 71-5274749. Fax: (31) 71-5274469. E-mail: j.bol{at}chem.leidenuniv.nl.

Journal of Virology, October 2003, p. 11284-11289, Vol. 77, No. 20
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.20.11284-11289.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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