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Journal of Virology, October 2003, p. 11268-11273, Vol. 77, No. 20
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.20.11268-11273.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of Endogenous Retroviruses in Sheep

Nikolai Klymiuk,1,2 Mathias Müller,2 Gottfried Brem,1,3 and Bernhard Aigner1,2*

ApoGene Biotechnologie, D-86567 Hilgertshausen, Germany,1 Institut für Tierzucht und Genetik, Veterinärmedizinische Universität Wien,2 Ludwig-Boltzmann-Institut für Immuno-, Zyto- und Molekulargenetische Forschung Wien, A-1210 Vienna, Austria3

Received 20 May 2003/ Accepted 21 July 2003

Endogenous retrovirus (ERV) sequences have been found in all mammals. In vitro and in vivo experiments revealed ERV activation and cross-species infection in several species. Sheep (Ovis aries) are used for various biotechnological purposes; however, they have not yet been comprehensively screened for ERV sequences. Therefore, the aim of the study was to classify the ERV sequences in the ovine genome (OERV) by analyzing the retroviral pro-pol sequences. Three OERV ß families and nine OERV {gamma} families were revealed. Novel open reading frames (ORF) in the amplified proviral fragment were found in one OERV ß family and two OERV {gamma} families. Hybrid OERV produced by putative recombination events were not detected. Quantitative analysis of the OERV sequences in the ovine genome revealed no relevant variations in the endogenous retroviral loads of different breeds. Expression analysis of different tissues from fetal and pregnant sheep detected mRNA from both gammaretrovirus families, showing ORF fragments. Thus, the release of retroviruses from sheep cells cannot be excluded.

* Corresponding author. Mailing address: Lehrstuhl für Molekulare Tierzucht und Biotechnologie, Moorversuchsgut, Hackerstr. 27, D-85764 Oberschleissheim, Germany. Phone: 49 89 315 732 22. Fax: 49 89 315 732 50. E-mail: b.aigner{at}gen.vetmed.uni-muenchen.de.

Journal of Virology, October 2003, p. 11268-11273, Vol. 77, No. 20
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.20.11268-11273.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Xiao, R., Park, K., Lee, H., Kim, J., Park, C. (2008). Identification and Classification of Endogenous Retroviruses in Cattle. J. Virol. 82: 582-587 [Abstract] [Full Text]  
  • Klymiuk, N., Muller, M., Brem, G., Aigner, B. (2006). Phylogeny, recombination and expression of porcine endogenous retrovirus {gamma}2 nucleotide sequences.. J. Gen. Virol. 87: 977-986 [Abstract] [Full Text]  


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