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Journal of Virology, January 2003, p. 1649-1652, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1649-1652.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Valency of Antibody Binding to Enveloped Virus Particles as Determined by Surface Plasmon Resonance

Sam A. Hardy and Nigel J. Dimmock*

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom

Received 26 July 2002/ Accepted 11 October 2002

A simple method is described for determining the valency of binding of immunoglobulin G to immobilized influenza A virus. Where there is a free Fab arm (monovalent binding), a second virus particle is captured. This is detected by surface plasmon resonance. The methodology should be applicable to all enveloped and nonenveloped viruses.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. Phone: 44 (0)2476-523593. Fax: 44 (0)2476 523568. E-mail: ndimmock{at}bio.warwick.ac.uk.

Journal of Virology, January 2003, p. 1649-1652, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1649-1652.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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