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Journal of Virology, January 2003, p. 1644-1648, Vol. 77, No. 2
0022-538X/03/$08.00+0 DOI: 10.1128/JVI.77.2.1644-1648.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Marjoleine A. Lucassen, Sophie Greve, Joyphi C. P. Thijssen, Willy J. M. Spaan, and Peter J. Bredenbeek*
Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
Received 10 June 2002/ Accepted 7 October 2002
Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3'-located conserved sequence 1 and an RNA sequence located in the 5' end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.
Present address: Central Institute for Animal Disease Control (CIDC) Lelystad, 8203 AA Lelystad, The Netherlands.
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