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Journal of Virology, January 2003, p. 1621-1625, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1621-1625.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Novel Viral Disease Control Strategy: Adenovirus Expressing Alpha Interferon Rapidly Protects Swine from Foot-and-Mouth Disease

Jarasvech Chinsangaram,{dagger} Mauro P. Moraes,{ddagger} Marla Koster, and Marvin J. Grubman*

Plum Island Animal Disease Center, USDA, ARS, NAA, Greenport, New York 11944

Received 22 July 2002/ Accepted 7 October 2002

We have previously shown that replication of foot-and-mouth disease virus (FMDV) is highly sensitive to alpha/beta interferon (IFN-{alpha}/ß). In the present study, we constructed recombinant, replication-defective human adenovirus type 5 vectors containing either porcine IFN-{alpha} or IFN-ß (Ad5-pIFN{alpha} or Ad5-pIFNß). We demonstrated that cells infected with these viruses express high levels of biologically active IFN. Swine inoculated with 109 PFU of a control Ad5 virus lacking the IFN gene and challenged 24 h later with FMDV developed typical signs of foot-and-mouth disease (FMD), including fever, vesicular lesions, and viremia. In contrast, swine inoculated with 109 PFU of Ad5-pIFN{alpha} were completely protected when challenged 24 h later with FMDV. These animals showed no clinical signs of FMD and no viremia and did not develop antibodies against viral nonstructural proteins, suggesting that complete protection from infection was achieved.

* Corresponding author. Mailing address: Plum Island Animal Disease Center, USDA, ARS, NAA, P.O. Box 848, Greenport, NY 11944. Phone: (631) 323-3329. Fax: (631) 323-3006. E-mail: mgrubman{at}piadc.ars.usda.gov.

{dagger} Present address: Therion Biologics Corporation, Cambridge, MA 02142.

{ddagger} Present address: Federal University of Vicosa, Department of Veterinary Virology, 36571-000 Vicosa-MG, Brazil.

Journal of Virology, January 2003, p. 1621-1625, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1621-1625.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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