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Journal of Virology, January 2003, p. 1329-1336, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1329-1336.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Negative-Strand Tospoviruses and Tenuiviruses Carry a Gene for a Suppressor of Gene Silencing at Analogous Genomic Positions

Etienne Bucher,1 Titia Sijen,2 Peter de Haan,3 Rob Goldbach,1 and Marcel Prins1*

Laboratory of Virology, Wageningen University, 6709 PD Wageningen,1 The Hubrecht Laboratory, 3584 CT Utrecht,2 Viruvation B.V., 2333 AL Leiden, The Netherlands3

Received 21 August 2002/ Accepted 8 October 2002

Posttranscriptional silencing of a green fluorescent protein (GFP) transgene in Nicotiana benthamiana plants was suppressed when these plants were infected with Tomato spotted wilt virus (TSWV), a plant-infecting member of the Bunyaviridae. Infection with TSWV resulted in complete reactivation of GFP expression, similar to the case for Potato virus Y, but distinct from that for Cucumber mosaic virus, two viruses known to carry genes encoding silencing suppressor proteins. Agrobacterium-based leaf injections with individual TSWV genes identified the NSS gene to be responsible for the RNA silencing-suppressing activity displayed by this virus. The absence of short interfering RNAs in NSS-expressing leaf sectors suggests that the tospoviral NSS protein interferes with the intrinsic RNA silencing present in plants. Suppression of RNA silencing was also observed when the NS3 protein of the Rice hoja blanca tenuivirus, a nonenveloped negative-strand virus, was expressed. These results indicate that plant-infecting negative-strand RNA viruses carry a gene for a suppressor of RNA silencing.

* Corresponding author. Mailing address: Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands. Phone: 31 317 483090. Fax: 31 317 484820. E-mail: marcel.prins{at}wur.nl.

Journal of Virology, January 2003, p. 1329-1336, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1329-1336.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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