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Tissue-Specific Replicating Capacity of a Chimeric Poliovirus That Carries the Internal Ribosome Entry Site of Hepatitis C Virus in a New Mouse Model Transgenic for the Human Poliovirus Receptor

Akiko Yanagiya,¹ Seii Ohka,¹ Noriyasu Hashida,¹ Masahito Okamura,¹ Choji Taya,² Nobuhiko Kamoshita,^1, Kuniko Iwasaki,³ Yukari Sasaki,³ Hiromichi Yonekawa,² and Akio Nomoto^1*

Department of Microbiology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033,¹ Department of Laboratory Animal Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613,² Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan³

Received 29 April 2003/ Accepted 10 July 2003

Nucleotides (nt) 108 to 742 of an infectious cDNA clone of poliovirus (PV) Mahoney strain, including the corresponding region of the internal ribosome entry site (IRES), was replaced by nt 28 to 710 of hepatitis C virus (HCV) cDNA corresponding to the whole HCV IRES. A chimeric PV (2A-369) was generated by transfecting mammalian cells with an RNA transcribed in vitro from the cDNA. To examine replicating capacity of virus 2A-369 in the brain and liver of a mouse model for poliomyelitis, a new mouse model (MPVRTg25-61) that is transgenic for human PV receptor (hPVR; CD155) was generated in order to obtain a higher expression level of hPVR in the liver than those of hPVRTg mouse lines generated by us so far. The transgene used was constructed by combining a putative regulatory region of the mouse PVR homolog and the whole structural region of the hPVR gene. Virus 2A-369 replicated well in the liver of MPVRTg25-61 but not in the brain, whereas control Mahoney virus replicated well both in the liver and in the brain. The data suggest that the HCV IRES works more efficiently in the liver than in the brain and that PV IRES works well both in the liver and in the brain. The results support the notion that tissue-specific activity of IRES may be reflected in tissue tropism of a virus whose specific translation initiation is driven by IRES, that is, an IRES-dependent virus tropism.

Present address: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139-4307.

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