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Journal of Virology, September 2003, p. 10162-10167, Vol. 77, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.18.10162-10167.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of White Spot Syndrome Virus Latency-Related Genes in Specific-Pathogen-Free Shrimps by Use of a Microarray

Siti Khadijah,1 Soek Ying Neo,2 M. S. Hossain,1 Lance D. Miller,2 S. Mathavan,2 and Jimmy Kwang1*

Animal Health Biotechnology, Temasek Life Sciences Laboratory, The National University of Singapore, Singapore 117604,1 Genome Institute of Singapore, Singapore 1175282

Received 30 April 2003/ Accepted 19 June 2003

To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.

* Corresponding author. Mailing address: Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore 117604. Phone: 65 6872 7473. Fax: 65 6872 7007. E-mail: kwang{at}tll.org.sg.

Journal of Virology, September 2003, p. 10162-10167, Vol. 77, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.18.10162-10167.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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