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Journal of Virology, September 2003, p. 10099-10105, Vol. 77, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.18.10099-10105.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Combinations of Two Capsid Regions Controlling Canine Host Range Determine Canine Transferrin Receptor Binding by Canine and Feline Parvoviruses

Karsten Hueffer,1 Lakshman Govindasamy,2 Mavis Agbandje-McKenna,2 and Colin R. Parrish1*

James A. Baker Institute, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853,1 Department of Biochemistry and Molecular Biology, Center for Structural Biology, The Brain Institute, College of Medicine, University of Florida, Gainesville, Florida 32610-02452

Received 10 March 2003/ Accepted 23 June 2003

Feline panleukopenia virus (FPV) and its host range variant, canine parvovirus (CPV), can bind the feline transferrin receptor (TfR), while only CPV binds to the canine TfR. Introducing two CPV-specific changes into FPV (at VP2 residues 93 and 323) endowed that virus with the canine TfR binding property and allowed canine cell infection, although neither change alone altered either property. In CPV the reciprocal changes of VP2 residue 93 or 323 to the FPV sequences individually resulted in modest reductions in infectivity for canine cells. Changing both residues in CPV to the FPV amino acids blocked the canine cell infection, but that virus was still able to bind the canine TfR at low levels. This shows that both CPV-specific changes control canine TfR binding but that binding is not always sufficient to mediate infection.

* Corresponding author. Mailing address: James A. Baker Institute for Animal Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853. Phone: (607) 256-5649. Fax: (607) 256-5608. E-mail: crp3{at}cornell.edu.

Journal of Virology, September 2003, p. 10099-10105, Vol. 77, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.18.10099-10105.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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