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Journal of Virology, September 2003, p. 9183-9191, Vol. 77, No. 17
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.17.9183-9191.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Adenovirus Type 11 Uses CD46 as a Cellular Receptor

Anna Segerman,1 John P. Atkinson,2 Marko Marttila,1 Veronica Dennerquist,1 Göran Wadell,1 and Niklas Arnberg1*

Department of Virology, Umeå University, SE-90185 Umeå, Sweden,1 Division of Rheumatology, Washington University School of Medicine, St. Louis, Missouri 631102

Received 13 January 2003/ Accepted 3 June 2003

The 51 human adenovirus serotypes are divided into six species (A to F). Many adenoviruses use the coxsackie-adenovirus receptor (CAR) for attachment to host cells in vitro. Species B adenoviruses do not compete with CAR-binding serotypes for binding to host cells, and it has been suggested that species B adenoviruses use a receptor other than CAR. Species B adenoviruses mainly cause disease in the respiratory tract, the eyes, and in the urinary tract. Here we demonstrate that adenovirus type 11 (Ad11; of species B) binds to Chinese hamster ovary (CHO) cells transfected with CD46 (membrane cofactor protein)-cDNA at least 10 times more strongly than to CHO cells transfected with cDNAs encoding CAR or CD55 (decay accelerating factor). Nonpermissive CHO cells were rendered permissive to Ad11 infection upon transfection with CD46-cDNA. Soluble Ad11 fiber knob but not Ad7 or Ad5 knob inhibited binding of Ad11 virions to CD46-transfected cells, and anti-CD46 antibodies inhibited both binding of and infection by Ad11. From these results we conclude that CD46 is a cellular receptor for Ad11.

* Corresponding author. Mailing address: Department of Virology, Umeå University, SE-90185 Umeå, Sweden. Phone: 46-90-785-2891. Fax: 46-90-129905. E-mail: niklas.arnberg{at}climi.umu.se.

Journal of Virology, September 2003, p. 9183-9191, Vol. 77, No. 17
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.17.9183-9191.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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