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Journal of Virology, August 2003, p. 9094-9098, Vol. 77, No. 16
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.16.9094-9098.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Vaccinia Virus Recombinant Expressing an 87-Kilodalton Polyprotein That Is Sufficient To Form Astrovirus-Like Particles

Rosa M. Dalton, Esperanza P. Pastrana, and Alicia Sánchez-Fauquier*

Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Madrid, Spain

Received 2 January 2003/ Accepted 22 May 2003

Human astrovirus is an important cause of acute gastroenteritis. We have generated, for the first time, a vaccinia virus recombinant expressing the astrovirus 87-kDa structural polyprotein. The results demonstrate that this expression results in the formation of virus-like particles in the absence of other astrovirus proteins and genomic RNA. The purified trypsin-activated virus-like particles strongly resemble the complete astrovirus particles.

* Corresponding author. Mailing address: Instituto de Salud Carlos III, Centro Nacional de Microbiología, Ctra. Majadahonda a Pozuelo Km.2, 28220 Madrid, Spain. Phone: 34 91 509 79 01, ext. 3655. Fax: 34 91 509 79 66. E-mail: asanchez{at}isciii.es.

Journal of Virology, August 2003, p. 9094-9098, Vol. 77, No. 16
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.16.9094-9098.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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