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Journal of Virology, August 2003, p. 9090-9093, Vol. 77, No. 16
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.16.9090-9093.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Expression of Immunogenic S1 Glycoprotein of Infectious Bronchitis Virus in Transgenic Potatoes

Ji-Yong Zhou,1 Jian-Xiang Wu,1 Li-Qin Cheng,1 Xiao-Juan Zheng,1 Hui Gong,1 Shao-Bin Shang,1 and En-Min Zhou2*

Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China,1 Department of Veterinary Diagnostic and Productive Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 500112

Received 19 February 2003/ Accepted 19 May 2003

The expression of infectious bronchitis virus (IBV) S1 glycoprotein in potatoes and its immunogenicity in mice and chickens were investigated. Potato plants were genetically transformed with a cDNA construct encoding the IBV S1 glycoprotein with the Agrobacterium system. Genomic DNA and mRNA analyses of the transformed plantlets confirmed the integration of the foreign cDNA into the potato genome, as well as its transcription. Mice and chickens vaccinated with the expressed IBV S1 glycoprotein produced antibodies that neutralized IBV infectivity. After three immunizations, vaccinated chickens were completely protected from virulent IBV infection. These results demonstrate that transgenic potatoes expressing IBV S1 glycoprotein can be used as a source of recombinant antigen for vaccine production.

* Corresponding author. Mailing address: Department of Veterinary Diagnostic and Production Animal Medicine, 2630 Vet. Med. Building, College of Veterinary Medicine, Iowa State University, Ames, IA 50011. Phone: (515) 294-4699. Fax: (515) 294-3654. E-mail: ezhou{at}iastate.edu.

Journal of Virology, August 2003, p. 9090-9093, Vol. 77, No. 16
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.16.9090-9093.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pogrebnyak, N., Golovkin, M., Andrianov, V., Spitsin, S., Smirnov, Y., Egolf, R., Koprowski, H. (2005). Severe acute respiratory syndrome (SARS) S protein production in plants: Development of recombinant vaccine. Proc. Natl. Acad. Sci. USA 102: 9062-9067 [Abstract] [Full Text]  


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