This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kelly, N. J.
Right arrow Articles by Morrow, C. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kelly, N. J.
Right arrow Articles by Morrow, C. D.

 Previous Article  |  Next Article 

Journal of Virology, August 2003, p. 8695-8701, Vol. 77, No. 16
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.16.8695-8701.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Selection of Retroviral Reverse Transcription Primer Is Coordinated with tRNA Biogenesis

Nathan J. Kelly,1 Matthew T. Palmer,2 and Casey D. Morrow2*

Department of Microbiology,1 Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 352942

Received 27 March 2003/ Accepted 21 May 2003

Initiation of retrovirus reverse transcription requires the selection of a tRNA primer from the intracellular milieu. To investigate the features of primer selection, a human immunodeficiency virus type 1 (HIV-1) and a murine leukemia virus (MuLV) were created that require yeast tRNAPhe to be supplied in trans for infectivity. Wild-type yeast tRNAPhe expressed in mammalian cells was transported to the cytoplasm and aminoacylated. In contrast, tRNAPhe without the D loop (tRNAPheD-) was retained within the nucleus and did not complement infectivity of either HIV-1 or MuLV; however, infectivity was restored when tRNAPheD- was directly transfected into the cytoplasm of cells. A tRNAPhe mutant (tRNAPheUUA) that did not have the capacity to be aminoacylated was transported to the cytoplasm and did complement infectivity of both HIV-1 and MuLV, albeit at a level less than that with wild-type tRNAPhe. Collectively, our results demonstrate that the tRNA primer captured by HIV-1 and MuLV occurs after nuclear export of tRNA and supports a model in which primer selection for retroviruses is coordinated with tRNA biogenesis at the intracellular site of protein synthesis.

* Corresponding author. Mailing address: Department of Cell Biology, University of Alabama at Birmingham, 802 Kaul Building, 720 20th St. South, Birmingham, AL 35294. Phone: (205) 934-5705. Fax: (205) 934-1580. E-mail: caseym{at}uab.edu.

Journal of Virology, August 2003, p. 8695-8701, Vol. 77, No. 16
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.16.8695-8701.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Palmer, M. T., Kirkman, R., Kosloff, B. R., Eipers, P. G., Morrow, C. D. (2007). tRNA Isoacceptor Preference prior to Retrovirus Gag-Pol Junction Links Primer Selection and Viral Translation. J. Virol. 81: 4397-4404 [Abstract] [Full Text]  
  • McCulley, A., Morrow, C. D. (2006). Complementation of Human Immunodeficiency Virus Type 1 Replication by Intracellular Selection of Escherichia coli Formula Supplied in trans. J. Virol. 80: 9641-9650 [Abstract] [Full Text]  
  • Kelly, N. J., Morrow, C. D. (2005). Structural Elements of the tRNA T{Psi}C Loop Critical for Nucleocytoplasmic Transport Are Important for Human Immunodeficiency Virus Type 1 Primer Selection. J. Virol. 79: 6532-6539 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»