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Journal of Virology, August 2003, p. 8588-8592, Vol. 77, No. 15
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.15.8588-8592.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Gammaherpesvirus Chemokine Binding Protein Binds to the N Terminus of CXCL8{dagger}

Louise M. C. Webb,1 Ian Clark-Lewis,2 and Antonio Alcami1*

Department of Medicine and Department of Pathology, Division of Virology, University of Cambridge, Cambridge, United Kingdom,1 Biomedical Research Centre and Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada2

Received 13 March 2003/ Accepted 7 May 2003

Viruses encode proteins that disrupt chemokine responses. The murine gammaherpesvirus 68 gene M3 encodes a chemokine binding protein (vCKBP-3) which has no sequence similarity to chemokine receptors but inhibits chemokine receptor binding and activity. We have used a panel of CXCL8 analogs to identify the structural requirements for CXCL8 to bind to vCKBP-3 in a scintillation proximity assay. Our data suggest that vCKBP-3 acts by mimicking the binding of chemokine receptors to CXCL8.

* Corresponding author. Mailing address: Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Level 5, Box 157, Hills Rd., Cambridge CB2 2QQ, United Kingdom. Phone: 44 1223 763403. Fax: 44 1223 330158. E-mail: aa258{at}mole.bio.cam.ac.uk.

{dagger} This paper is dedicated to the memory of Ian Clark-Lewis (1955 to 2002).

Journal of Virology, August 2003, p. 8588-8592, Vol. 77, No. 15
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.15.8588-8592.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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