This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kobayashi, K. Articles by Hohn, T. Search for Related Content PubMed PubMed Citation Articles by Kobayashi, K. Articles by Hohn, T.

Dissection of Cauliflower Mosaic Virus Transactivator/Viroplasmin Reveals Distinct Essential Functions in Basic Virus Replication

Friedrich Miescher Institute, CH-4002 Basel, Switzerland

Received 3 March 2003/ Accepted 16 May 2003

Cauliflower mosaic virus (CaMV) transactivator/viroplasmin (Tav) is an essential multifunctional viral protein. Dissection of Tav by deletion mutagenesis revealed that the central region is essential for CaMV replication in single cells but that the N- and C-terminal parts are not. Strains with mutations in the central region were defective in the translational transactivator function and could be complemented by coexpressing Gag (capsid protein precursor) and Pol (polyprotein with protease, reverse transcriptase, and RNase H activity) from separate monocistronic plasmids. In contrast, total omission of Tav was only partially complemented by Gag and Pol overexpression from separate plasmids. These results indicate that CaMV basic replication requires both Tav-activated polycistronic translation and some posttranslational function(s) of Tav that is not affected by the deletions in the central region of Tav.

This article has been cited by other articles:

• Love, A. J., Laird, J., Holt, J., Hamilton, A. J., Sadanandom, A., Milner, J. J. (2007). Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing. J. Gen. Virol. 88: 3439-3444 [Abstract] [Full Text]  
• Stavolone, L., Villani, M. E., Leclerc, D., Hohn, T. (2005). A coiled-coil interaction mediates cauliflower mosaic virus cell-to-cell movement. Proc. Natl. Acad. Sci. USA 102: 6219-6224 [Abstract] [Full Text]  
• Haas, M., Geldreich, A., Bureau, M., Dupuis, L., Leh, V., Vetter, G., Kobayashi, K., Hohn, T., Ryabova, L., Yot, P., Keller, M. (2005). The Open Reading Frame VI Product of Cauliflower mosaic virus Is a Nucleocytoplasmic Protein: Its N Terminus Mediates Its Nuclear Export and Formation of Electron-Dense Viroplasms. Plant Cell 17: 927-943 [Abstract] [Full Text]