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Journal of Virology, July 2003, p. 8141-8146, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.8141-8146.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Biphasic DNA Synthesis in Spumaviruses

Olivier Delelis,1 Ali Saïb,2 and Pierre Sonigo1*

Département des Maladies Infectieuses, Institut Cochin, INSERM U 567 et CNRS UMR 8104, 75014 Paris,1 CNRS UPR 9051, Hôpital Saint-Louis, 75010 Paris, France2

Received 6 December 2002/ Accepted 15 April 2003

Spumaviruses are complex retroviruses whose replication cycle resembles that of hepadnaviruses, especially by a late-occurring reverse transcription step. The possible existence of an early reverse transcription as observed in other retroviruses was not documented. Using real-time quantitative PCR, we addressed directly the kinetics of DNA synthesis during spumavirus infection. An early phase of viral DNA synthesis developed until 3 h postinfection, followed by a second phase, culminating 10 h postinfection. Both phases were abolished by the reverse transcriptase inhibitor 3'-azido-3'-deoxythymidine. Similar to other retroviruses, circular forms of viral DNA harboring two long terminal repeats were mainly found in the nucleus of infected cells. Interestingly, a fraction of these circular forms were detected in the cytoplasm and in extracellular virions, a feature shared with hepadnaviruses. Combined with packaging of both viral DNA and RNA genomes in virions, early and late reverse transcription might allow spumavirus to maximize its genome replication.

* Corresponding author. Mailing address: Département des Maladies Infectieuses, Institut Cochin, INSERM U 567 et CNRS UMR 8104, 22 rue Méchain, 75014 Paris, France. Phone: 33 1 40 51 64 13. Fax: 33 1 40 51 64 30. E-mail: sonigo{at}cochin.inserm.fr.

Journal of Virology, July 2003, p. 8141-8146, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.8141-8146.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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