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Journal of Virology, July 2003, p. 8039-8047, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.8039-8047.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Vesicular Stomatitis Virus Glycoprotein Is a Determinant of Pathogenesis in Swine, a Natural Host

Isidoro Martinez,¹ Luis L. Rodriguez,^2* Carlos Jimenez,^2, Steven J. Pauszek,² and Gail W. Wertz¹

Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294,¹ Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, New York 11944²

Received 7 February 2003/ Accepted 11 April 2003

There are two major serotypes of vesicular stomatitis virus (VSV), Indiana (VSIV) and New Jersey (VSNJV). We recovered recombinant VSIVs from engineered cDNAs that contained either (i) one copy of the VSIV G gene (VSIV-G^I); (ii) two copies of the G gene, one from each serotype (VSIV-G^NJG^I); or (iii) a single copy of the G^NJ gene instead of the G^I gene (VSIV-G^NJ). The recombinant viruses expressed the appropriate glycoproteins, incorporated them into virions, and were neutralized by antibodies specific for VSIV (VSIV-G^I), VSNJV (VSIV-G^NJ), or both (VSIV-G^NJG^I), according to the glycoprotein(s) they expressed. All recombinant viruses grew to similar titers in cell culture. In mice, VSIV-G^NJ and VSIV-G^NJG^I were attenuated. However, in swine, a natural host for VSV, the G^NJ glycoprotein-containing viruses caused more severe lesions and replicated to higher titers than the parental virus, VSIV-G^I. These observations implicate the glycoprotein as a determinant of VSV virulence in a natural host and emphasize the differences in VSV pathogenesis between mice and swine.

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* Corresponding author. Mailing address: Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Orient Point, Long Island, P.O. Box 848, Greenport, NY 11944-0848. Phone: (631) 323-3364. Fax: (631) 323-2507. E-mail: lrodriguez{at}piadc.ars.usda.gov.

Present address: Tropical Disease Research Program, School of Veterinary Medicine, Universidad Nacional, Heredia, Costa Rica.

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Journal of Virology, July 2003, p. 8039-8047, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.8039-8047.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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