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Journal of Virology, July 2003, p. 7814-7819, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.7814-7819.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

An Infectious Clone of Woolly Monkey Hepatitis B Virus

Robert E. Lanford,1,2* Deborah Chavez,1 Azeneth Barrera,1,2 and Kathleen M. Brasky3

Department of Virology and Immunology,1 Department of Comparative Medicine, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227,3 Department of Microbiology, University of Texas Health Science Center, San Antonio, Texas 782292

Received 30 January 2003/ Accepted 30 April 2003

Members of the Hepadnaviridae family have been isolated from birds, rodents, and primates. A new hepadnavirus isolated from the woolly monkey, a New World primate, is phylogenetically distinct from other primate isolates. An animal model has been established for woolly monkey hepatitis B virus (WMHBV) by using spider monkeys, since woolly monkeys are endangered. In this study, a greater-than-genome length construct was prepared without amplification by using covalently closed circular DNA extracted from the liver of an infected woolly monkey. Transfection of the human liver cell line Huh7 with WMHBV DNA resulted in the production of viral transcripts, DNA replicative intermediates, and secreted virions at levels similar to those obtained with an infectious human HBV clone, demonstrating that the host range restriction of WMHBV is not at the level of genome replication. WMHBV particles from the medium of transfected cultures initiated an infection in a spider monkey similar to that obtained with virions derived from woolly monkey serum. In an attempt to adapt the virus for higher levels of replication in spider monkeys, immunosuppressed and newborn animals were inoculated. Neither procedure produced persistent infections, and the level of viral replication remained several logs lower than that observed in persistently infected woolly monkeys. These data demonstrate the production of an infectious clone for WMHBV and extend the characterization of the spider monkey animal model.

* Corresponding author. Mailing address: Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, 7620 N.W. Loop 410, San Antonio, TX 78227. Phone: (210) 258-9442. Fax: (210) 670-3229. E-mail: rlanford{at}icarus.sfbr.org.

Journal of Virology, July 2003, p. 7814-7819, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.7814-7819.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mason, W. S., Low, H.-C., Xu, C., Aldrich, C. E., Scougall, C. A., Grosse, A., Clouston, A., Chavez, D., Litwin, S., Peri, S., Jilbert, A. R., Lanford, R. E. (2009). Detection of Clonally Expanded Hepatocytes in Chimpanzees with Chronic Hepatitis B Virus Infection. J. Virol. 83: 8396-8408 [Abstract] [Full Text]  
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