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Journal of Virology, July 2003, p. 7663-7668, Vol. 77, No. 13
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.13.7663-7668.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Vaccine-Induced Immunity in Baboons by Using DNA and Replication-Incompetent Adenovirus Type 5 Vectors Expressing a Human Immunodeficiency Virus Type 1 gag Gene

Danilo R. Casimiro,1* Aimin Tang,1 Ling Chen,1 Tong-Ming Fu,1 Robert K. Evans,2 Mary-Ellen Davies,1 Daniel C. Freed,1 William Hurni,3 Jose M. Aste-Amezaga,3 Liming Guan,1 Romnie Long,1 Lingyi Huang,1 Virginia Harris,1 Denise K. Nawrocki,1 Henryk Mach,2 Robert D. Troutman,2 Lynne A. Isopi,2 Krishna K. Murthy,4 Karen Rice,4 Keith A. Wilson,1 David B. Volkin,1 Emilio A. Emini,1 and John W. Shiver1

Departments of Viral Vaccine Research,1 Vaccine Pharmaceutical Research and Development,2 Virus and Cell Biology, Merck Research Laboratories, West Point, Pennsylvania 19486,3 Department of Virology and Immunology, Southwest Foundation for Medical Research, San Antonio, Texas 782454

Received 9 December 2002/ Accepted 7 April 2003

The cellular immunogenicity of formulated plasmid DNA and replication-defective human adenovirus serotype 5 (Ad5) vaccine vectors expressing a codon-optimized human immunodeficiency virus type 1 gag gene was examined in baboons. The Ad5 vaccine was capable of inducing consistently strong, long-lived CD8+-biased T-cell responses and in vitro cytotoxic activities. The DNA vaccine-elicited immune responses were weaker than those elicited by the Ad5 vaccine and highly variable; formulation with chemical adjuvants led to moderate increases in the levels of Gag-specific T cells. Increasing the DNA-primed responses with booster doses of either Ad5 or modified vaccinia virus Ankara vaccines suggests a difference in the relative levels of cytotoxic and helper responses. The implications of these results are discussed.

* Corresponding author. Mailing address: Department of Viral Vaccine Research, Merck & Co., WP26-145, 770 Sumneytown Pike, West Point, PA 19486. Phone: (215) 652-3129. Fax: (215) 652-7320. E-mail: danilo_casimiro{at}merck.com.

Journal of Virology, July 2003, p. 7663-7668, Vol. 77, No. 13
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.13.7663-7668.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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