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Journal of Virology, July 2003, p. 7502-7509, Vol. 77, No. 13
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.13.7502-7509.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Replication and IRES-Dependent Translation Are Both Affected by Core Coding Sequences in Subgenomic GB Virus B Replicons

Maura Pizzuti, Amedeo De Tomassi, and Cinzia Traboni*

Istituto di Ricerche di Biologia Molecolare P. Angeletti, 00040 Pomezia, Rome, Italy

Received 26 December 2002/ Accepted 31 March 2003

The yield of G418-resistant Huh7 cell clones bearing subgenomic dicistronic GB virus B (GBV-B) is significantly affected by the insertion of a portion of the viral core gene between the GBV-B 5' untranslated region and the exogenous neomycin phosphotransferase selector gene (A. De Tomassi, M. Pizzuti, R. Graziani, A. Sbardellati, S. Altamura, G. Paonessa, and C. Traboni, J. Virol. 76:7736-7746, 2002). In this report, we have dissected this phenomenon, examining the effects of the insertion of core sequences of different lengths on GBV-B IRES-dependent translation and RNA replication by using experimental approaches aimed at analyzing these two aspects independently. The results achieved indicate that an enhancement of translation efficiency does occur and that it correlates with the length of the inserted core sequences. Interestingly, the insertion of these sequences also has a direct similar effect on the efficiency of replication of the GBV-B replicon. These results suggest that in GBV-B replicon RNA and potentially in the complete viral genome, the core coding sequences not only are part of the IRES but also take part in the replication process, independently of the presence of the corresponding whole protein.

* Corresponding author. Mailing address: Istituto di Ricerche di Biologia Molecolare P. Angeletti, Via Pontina Km 30,600, 00040 Pomezia, Rome, Italy. Phone: (39.06) 91093241. Fax: (39.06) 91093654. E-mail: cinzia_traboni{at}merck.com.

Journal of Virology, July 2003, p. 7502-7509, Vol. 77, No. 13
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.13.7502-7509.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Piron, M., Beguiristain, N., Nadal, A., Martinez-Salas, E., Gomez, J. (2005). Characterizing the function and structural organization of the 5' tRNA-like motif within the hepatitis C virus quasispecies. Nucleic Acids Res 33: 1487-1502 [Abstract] [Full Text]  
  • De Tomassi, A., Pizzuti, M., Traboni, C. (2003). Hep3B Human Hepatoma Cells Support Replication of the Wild-Type and a 5'-End Deletion Mutant GB Virus B Replicon. J. Virol. 77: 11875-11881 [Abstract] [Full Text]  


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