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Journal of Virology, July 2003, p. 7486-7491, Vol. 77, No. 13
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.13.7486-7491.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Intranasal Immunization of Guinea Pigs with an Immunodominant Foot-and-Mouth Disease Virus Peptide Conjugate Induces Mucosal and Humoral Antibodies and Protection against Challenge{dagger}

D. Fischer,1,{ddagger} D. Rood,1 R. W. Barrette,1 A. Zuwallack,1 E. Kramer,2 F. Brown,2 and L. K. Silbart1*

Center of Excellence for Vaccine Research, University of Connecticut, Storrs, Connecticut,1 U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, New York2

Received 4 October 2002/ Accepted 18 March 2003

Guinea pigs immunized intranasally with a keyhole limpet hemocyanin-linked peptide, corresponding to the prominent G-H loop of the VP1 protein of foot-and-mouth disease virus, raised substantial levels of antipeptide and virus-neutralizing antibodies in sera and of peptide-specific secretory immunoglobulin A in nasal secretions. In groups of animals immunized intranasally without adjuvant, 86 percent were fully protected upon challenge with homotypic virus. Surprisingly, animals given the peptide conjugates plus the mucosal adjuvant cholera toxin were afforded only partial protection in that primary lesions were observed in most animals, although spread to other feet was prevented. These results indicate that intranasal inoculation with the peptide offers a potential route of vaccination against foot-and-mouth disease and may be useful for eliciting protection in the upper respiratory tracts of susceptible animals.

* Corresponding author. Mailing address: University of Connecticut, Center of Excellence for Vaccine Research, 1390 Storrs Rd., Unit-4163, Storrs, CT 06279-4163. Phone: (860) 486-6073. Fax: (860) 486-5067. E-mail: lsilbart{at}canr.uconn.edu.

{dagger} Agricultural Experiment Station publication 2126, Storrs Agricultural Experiment Station, Storrs, Conn. Center of Excellence for Vaccine Research publication 082.

{ddagger} Present address: Guelph University School of Veterinary Medicine, Guelph, Ontario, Canada.

Journal of Virology, July 2003, p. 7486-7491, Vol. 77, No. 13
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.13.7486-7491.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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