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Journal of Virology, July 2003, p. 7254-7260, Vol. 77, No. 13
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.13.7254-7260.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Interaction of Rotaviruses with Hsc70 during Cell Entry Is Mediated by VP5

Selene Zárate, Mariela A. Cuadras, Rafaela Espinosa, Pedro Romero, Karla O. Juárez, Minerva Camacho-Nuez, Carlos F. Arias, and Susana López*

Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, Mexico

Received 7 January 2003/ Accepted 8 April 2003

Rotavirus infection seems to be a multistep process in which the viruses are required to interact with several cell surface molecules to enter the cell. The virus spike protein VP4, which is cleaved by trypsin into two subunits, VP5 and VP8, is involved in some of these interactions. We have previously shown that the neuraminidase-sensitive rotavirus strain RRV initially attaches to a sialic acid-containing cell molecule through the VP8 subunit of VP4 and subsequently interacts with integrin {alpha}2ß1 through VP5. After these initial contacts, the virus interacts with at least two additional proteins located at the cell surface, the integrin {alpha}vß3 and the heat shock cognate protein Hsc70. In this work, we have shown that rotavirus RRV and its neuraminidase-resistant variant nar3 interact with Hsc70 through a VP5 domain located between amino acids 642 and 658 of the protein. This conclusion is based on the observation that a recombinant protein comprising the 300 carboxy-terminal amino acids of VP5 binds specifically to Hsc70 and a synthetic peptide containing amino acids 642 to 658 competes with the binding of the RRV and nar3 viruses to the heat shock protein. The VP5 peptide also competed with the binding to Hsc70 of the recombinant VP5 protein, and an antibody to Hsc70 reduced the binding of the recombinant protein to the surface of MA104 cells. The fact that the synthetic peptide blocks the infectivity of rotaviruses RRV and nar3 but not their binding to cells indicates that the interaction of VP5 with Hsc70 most probably occurs at a postattachment step during the virus entry process.

* Corresponding author. Mailing address: Departamento de Génetica del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico. Phone: (52) (73) 291615. Fax: (52) (73) 172388. E-mail: susana{at}ibt.unam.mx.

Journal of Virology, July 2003, p. 7254-7260, Vol. 77, No. 13
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.13.7254-7260.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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