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Journal of Virology, June 2003, p. 6979-6987, Vol. 77, No. 12
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.12.6979-6987.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Varicella-Zoster Virus DNA in Cells Isolated from Human Trigeminal Ganglia

Myron J. Levin,1* Guang-Yun Cai,1 Michael D. Manchak,1 and Lewis I. Pizer2

Section of Pediatric Infectious Diseases,1 Department of Microbiology, School of Medicine, University of Colorado, Denver, Colorado 802622

Received 21 November 2002/ Accepted 17 March 2003

To determine the type of cell(s) that contain latent varicella-zoster virus (VZV) DNA, we prepared pure populations of neurons and satellite cells from trigeminal ganglia of 18 humans who had previously had a VZV infection. VZV DNA was present in 34 of 2,226 neurons (1.5%) and in none of 20,700 satellite cells. There was an average of 4.7 (range of 2 to 9) copies of VZV DNA per latently infected neuron. Latent VZV DNA was primarily present in large neurons, whereas the size distribution of herpes simplex virus DNA was markedly different.

* Corresponding author. Mailing address: University of Colorado Health Sciences Center, 4200 East Ninth Ave., C-227, Denver, CO 80262. Phone: (303) 315-4620. Fax: (303) 315-7909. E-mail: myron.levin{at}uchsc.edu.

Journal of Virology, June 2003, p. 6979-6987, Vol. 77, No. 12
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.12.6979-6987.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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