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Journal of Virology, June 2003, p. 6562-6566, Vol. 77, No. 11
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.11.6562-6566.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Extensive Cross-Reactivity of CD4+ Adenovirus-Specific T Cells: Implications for Immunotherapy and Gene Therapy

Bianca Heemskerk,1* Louise A. Veltrop-Duits,1 Tamara van Vreeswijk,1 Monique M. ten Dam,1 Sebastiaan Heidt,1 Rene E. M. Toes,2 Maarten J. D. van Tol,1 and Marco W. Schilham1

Department of Pediatrics,1 Department of Rheumatology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands2

Received 6 December 2002/ Accepted 4 March 2003

Adenovirus (Ad)-specific T-cell responses in healthy adult donors were investigated. Ad5, inactivated by methylene blue plus visible light, induced proliferation and gamma interferon (IFN-{gamma}) production in peripheral blood mononuclear cells of the majority of donors. Responding T cells were CD4+ and produced IFN-{gamma} upon restimulation with infectious Ad5 and Ads of different subgroups. T-cell clones showed distinct cross-reactivity patterns recognizing Ad serotypes from either one subgroup (C), two subgroups (B and C), or three subgroups (A, B, and C). This cross-reactivity of Ad-specific T cells has relevance both for Ad-based gene therapy protocols, as well as for the feasibility of T-cell-mediated adoptive immunotherapy in recipients of an allogeneic stem cell transplantation.

* Corresponding author. Mailing address: Department of Pediatrics, P3-P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: 31-71-5263626. Fax: 31-71-5266876. E-mail: B.Heemskerk{at}lumc.nl.

Journal of Virology, June 2003, p. 6562-6566, Vol. 77, No. 11
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.11.6562-6566.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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