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Journal of Virology, June 2003, p. 6562-6566, Vol. 77, No. 11
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.11.6562-6566.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Extensive Cross-Reactivity of CD4⁺ Adenovirus-Specific T Cells: Implications for Immunotherapy and Gene Therapy

Department of Pediatrics,¹ Department of Rheumatology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands²

Received 6 December 2002/ Accepted 4 March 2003

Adenovirus (Ad)-specific T-cell responses in healthy adult donors were investigated. Ad5, inactivated by methylene blue plus visible light, induced proliferation and gamma interferon (IFN-) production in peripheral blood mononuclear cells of the majority of donors. Responding T cells were CD4⁺ and produced IFN- upon restimulation with infectious Ad5 and Ads of different subgroups. T-cell clones showed distinct cross-reactivity patterns recognizing Ad serotypes from either one subgroup (C), two subgroups (B and C), or three subgroups (A, B, and C). This cross-reactivity of Ad-specific T cells has relevance both for Ad-based gene therapy protocols, as well as for the feasibility of T-cell-mediated adoptive immunotherapy in recipients of an allogeneic stem cell transplantation.

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