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Journal of Virology, June 2003, p. 6546-6550, Vol. 77, No. 11
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.11.6546-6550.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multiple Epstein-Barr Virus Infections in Healthy Individuals

Dennis M. Walling,1,2* Abigail L. Brown,1 Wiguins Etienne,1 Wendy A. Keitel,3,4 and Paul D. Ling4

Division of Infectious Diseases, Department of Internal Medicine,1 Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston,2 Department of Medicine,3 Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas4

Received 2 December 2002/ Accepted 7 March 2003

We employed a newly developed genotyping technique with direct representational detection of LMP-1 gene sequences to study the molecular epidemiology of Epstein-Barr virus (EBV) infection in healthy individuals. Infections with up to five different EBV genotypes were found in two of nine individuals studied. These results support the hypothesis that multiple EBV infections of healthy individuals are common. The implications for the development of an EBV vaccine are discussed.


* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Internal Medicine, The University of Texas Medical Branch at Galveston, 301 University Blvd., Galveston, TX 77555-0435. Phone: (409) 747-2361. Fax: (409) 772-6527. E-mail: dwalling{at}utmb.edu.


Journal of Virology, June 2003, p. 6546-6550, Vol. 77, No. 11
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.11.6546-6550.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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