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Journal of Virology, June 2003, p. 6314-6321, Vol. 77, No. 11
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.11.6314-6321.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Unique Vertex of Bacterial Virus PRD1 Is Connected to the Viral Internal Membrane

Department of Biosciences and Institute of Biotechnology, Biocenter 2, FIN-00014 University of Helsinki, Finland

Received 27 November 2002/ Accepted 11 March 2003

Icosahedral double-stranded DNA (dsDNA) bacterial viruses are known to package their genomes into preformed procapsids via a unique portal vertex. Bacteriophage PRD1 differs from the more commonly known icosahedral dsDNA phages in that it contains an internal lipid membrane. The packaging of PRD1 is known to proceed via preformed empty capsids. Now, a unique vertex has been shown to exist in PRD1. We show in this study that this unique vertex extends to the virus internal membrane via two integral membrane proteins, P20 and P22. These small membrane proteins are necessary for the binding of the putative packaging ATPase P9, via another capsid protein, P6, to the virus particle.

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