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Journal of Virology, May 2003, p. 6076-6081, Vol. 77, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.10.6076-6081.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Two Major Histocompatibility Complex Class I-Restricted Epitopes of the Borna Disease Virus p10 Protein Identified by Cytotoxic T Lymphocytes Induced by DNA-Based Immunization

Yoshio Hashimoto,^1, Horng-Shen Chen,¹ Cynthia Cunningham,² Tahir H. Malik,³ and Patrick K. Lai^1*

Department of Bioscience, Salem International University, Salem, West Virginia 26426-0500,¹ Department of Microbiology, Immunology & Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia 26506-9177 ,² Laboratory of Pediatric and Respiratory Viral Diseases, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, Maryland 20892³

Received 9 September 2002/ Accepted 18 February 2003

Borna disease virus (BDV) infection of Lewis rats is the most studied animal model of Borna disease, an often fatal encephalomyelitis. In this experimental model, BDV-specific CD8⁺ cytotoxic T lymphocytes (CTLs) play a prominent role in the immunopathogenesis of infection by the noncytolytic, persistent BDV. Of the six open reading frames of BDV, CTLs to BDV X (p10) and the L-polymerase have never been studied. In this study, we used plasmid immunization to investigate the CTL response to BDV X and N. Plasmid-based immunization was a potent CTL inducer in Lewis rats. Anti-X CTLs were primed by a single injection of the p10 cDNA. Two codominant p10 epitopes, M₁SSDLRLTLL₁₀ and T₈LLELVRRL₁₆, associated with the RT1.A^l major histocompatibility complex class I molecules of the Lewis rats, were identified. In addition, immunization with a BDV p40-expressing plasmid confirmed the previously reported RT1.A^l-restricted A₂₃₀SYAQMTTY₂₃₈ peptide as the CTL target for BDV N. In contrast to the CTL responses, plasmid vaccination was a poor inducer of an antibody response to p10. Three injections of a recombinant eukaryotic expression plasmid of BDV p10 were needed to generate a weak anti-p10 immunoglobulin M response. However, the antibody response could be optimized by a protein boost after priming with cDNA.

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* Corresponding author. Mailing address: Department of Bioscience, Salem International University, 223 West Main St., Salem, WV 26426-0500. Phone: (304) 782-5575. Fax: (304) 782-5579. E-mail: lai{at}salemiu.edu.

Present address: Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

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Journal of Virology, May 2003, p. 6076-6081, Vol. 77, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.10.6076-6081.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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