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Journal of Virology, May 2003, p. 6066-6069, Vol. 77, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.10.6066-6069.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

RNA Interference of Human Papillomavirus Type 18 E6 and E7 Induces Senescence in HeLa Cells{dagger}

Allison H. S. Hall1 and Kenneth A. Alexander1,2*

Department of Molecular Genetics and Microbiology,1 Division of Pediatric Infectious Disease, Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 277102

Received 23 December 2002/ Accepted 27 February 2003

The human papillomavirus oncoproteins E6 and E7 promote cell proliferation and contribute to carcinogenesis by interfering with the activities of cellular tumor suppressors. We used a small interfering RNA molecule targeting the E7 region of the bicistronic E6 and E7 mRNA to induce RNA interference, thereby reducing expression of E6 and E7 in HeLa cells. RNA interference of E6 and E7 also inhibited cellular DNA synthesis and induced morphological and biochemical changes characteristic of cellular senescence. These results demonstrate that reducing E6 and E7 expression is sufficient to cause HeLa cells to become senescent.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-9590. Fax: (919) 684-8735. E-mail: alexa005{at}mc.duke.edu.

{dagger} Dedicated to the memory of Colleen Haberstroh.

Journal of Virology, May 2003, p. 6066-6069, Vol. 77, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.10.6066-6069.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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